製品の概要

  • 製品名Anti-Dnmt3a antibody
    Dnmt3a 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Dnmt3a
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Cow, Dog, Physarum polycephalum
  • 免疫原

    Synthetic peptide:

    DKNATKAGDDEPEY

    by a Cysteine residue linker, corresponding to internal sequence amino acids 271-284 of Human Dnmt3a (NP_072046.2; NP_715640.2; NP_783329.1).

  • ポジティブ・コントロール
    • Human and Rat liver lysates.
  • 特記事項


    ab113430 is expected to recognize reported isoforms a (NP_072046.2) and isoform b (NP_715640.2). Reported variants represent identical protein: NP_072046.2, NP_783328.1. ab113430 is not expected to recognize Dnmt3B.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab113430 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 一次抗体 備考ab113430 is expected to recognize reported isoforms a (NP_072046.2) and isoform b (NP_715640.2). Reported variants represent identical protein: NP_072046.2, NP_783328.1. ab113430 is not expected to recognize Dnmt3B.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa). Primary incubation time: 1 hour.

ターゲット情報

  • 機能Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • 組織特異性Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • 配列類似性Belongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • ドメインThe PWWP domain is essential for targeting to pericentric heterochromatin.
  • 翻訳後修飾Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • 細胞内局在Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3 alpha antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • OTTHUMP00000201149 antibody
    • TBRS antibody
    see all

Anti-Dnmt3a antibody 画像

  • Anti-Dnmt3a antibody (ab113430) at 0.5 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 102 kDa

Anti-Dnmt3a antibody (ab113430) 使用論文

ab113430 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab113430.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"