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Bacteria expressed HIS-tag recombinant mouse Dnmt3a.
Our Abpromise guarantee covers the use of ab13888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Use at a concentration of 2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 102 kDa). Dnmt3a Transfected and Untransfected Cell Lysate (ab90154) is the recommended positive control for WB.|
|IHC-P||Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration.|
|ICC||Use a concentration of 5 µg/ml.|
Immuno-staining of Dnmt3a using ab13888 at 5 ug/ml dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.