Rabbit polyclonal to Dnmt2
This antibody will recognise the splice isoforms A, B, and C.
A KLH conjugated synthetic peptide selected from the human Dnmt2 sequence within amino acids 300~400.
Human hepatocarcinoma tissue, Jurkat cell lysate and mouse liver tissue lysate.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralised immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/50 - 1/100.
WB: 1/100 - 1/500. Detects a band of approximately 55 kDa plus a range of other bands (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
Belongs to the C5-methyltransferase family.
Information by UniProt
DmMT 2 antibody
Western blot - Anti-Dnmt2 antibody (ab71015)
All lanes :
Anti-Dnmt2 antibody (ab71015) at 1/100 dilution
Lane 1 :
Jurkat cell lysate
Lane 2 :
Mouse liver tissue lysate
Lysates/proteins at 12.5 µg per lane.
Secondary All lanes :
Predicted band size:
Observed band size:
55 kDa (
why is the actual band size different from the predicted?
Note: bands in the tissue lysate are isoforms.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt2 antibody (ab71015)
Formalin fixed, paraffin embedded human hepatocarcinoma tissue reacted with ab71015 at a 1/50 dilution to stain Dnmt2. ab71015 was peroxidase conjugated to the secondary antibody, followed by DAB staining.
has not yet been referenced specifically in any publications.
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