製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Dnmt2
  • 特異性
    This antibody will recognise the splice isoforms A, B, and C.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the human Dnmt2 sequence within amino acids 300~400.

  • ポジティブ・コントロール
    • Human hepatocarcinoma tissue, Jurkat cell lysate and mouse liver tissue lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralised immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報
    ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 55 kDa plus a range of other bands (predicted molecular weight: 45 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp).
    • 組織特異性
      Ubiquitous. Higher expression in testis, ovary and thymus and at much lower levels in spleen, prostate, colon, small intestine, and peripheral blood leukocytes.
    • 配列類似性
      Belongs to the C5-methyltransferase family.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • dDNMT antibody
      • DmMT 2 antibody
      • DmMT2 antibody
      • DNA (cytosine 5 ) methyltransferase 2 antibody
      • DNA (cytosine 5) methyltransferase like protein 2 antibody
      • DNA (cytosine-5)-methyltransferase-like protein 2 antibody
      • DNA 5 cytosine methyltransferase antibody
      • DNA methyltransferase 2 antibody
      • DNA methyltransferase homolog HsaIIP antibody
      • DNA MTase homolog HsaIIP antibody
      • Dnmt 2 antibody
      • Dnmt2 antibody
      • M.HsaIIP antibody
      • MHsaIIP antibody
      • nmt 2 antibody
      • nmt2 antibody
      • OTTHUMP00000045198 antibody
      • PuMet antibody
      • RNMT 1 antibody
      • RNMT1 antibody
      • TRDMT 1 antibody
      • TRDMT_HUMAN antibody
      • TRDMT1 antibody
      • tRNA (cytosine 5 ) methyltransferase antibody
      • tRNA (cytosine(38)-C(5))-methyltransferase antibody
      • tRNA aspartic acid methyltransferase 1 antibody
      • tRNA aspartic acid methyltransferase 1 variant 1 antibody
      • tRNA aspartic acid methyltransferase 1 variant 2 antibody
      • tRNA aspartic acid methyltransferase 1 variant 3 antibody
      • tRNA aspartic acid methyltransferase 1 variant 4 antibody
      • tRNA aspartic acid methyltransferase 1 variant 5 antibody
      • tRNA aspartic acid methyltransferase 1 variant 8 antibody
      see all

    Anti-Dnmt2 antibody 画像

    • All lanes : Anti-Dnmt2 antibody (ab71015) at 1/100 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Mouse liver tissue lysate

      Lysates/proteins at 12.5 µg per lane.

      Secondary
      HRP-anti-rabbit

      Predicted band size : 45 kDa
      Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    • Formalin fixed, paraffin embedded human hepatocarcinoma tissue reacted with ab71015 at a 1/50 dilution to stain Dnmt2. ab71015 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

    Anti-Dnmt2 antibody (ab71015) 使用論文

    ab71015 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab71015.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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