製品の概要

  • 製品名Anti-Dnmt1 antibody - ChIP Grade
    Dnmt1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Dnmt1 - ChIP Grade
  • アプリケーション適用あり: WB, IP, ICC/IF, ChIPmore details
  • 種交差性
    交差種: Mouse, Human, Xenopus laevis
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1037-1386 of Mouse Dnmt1, purified by SDS-PAGE.

  • ポジティブ・コントロール
    • Murine erythrolekemia cells MEL11A2

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 183 kDa.
IP Use at an assay dependent dilution. Especially good for denatured protein.
ICC/IF 1/2000 - 1/5000. Background can be lowered by treatment of sample with SDS/TritonX-100 mixture (1:5 weight ratio).
ChIP Use at an assay dependent dilution.

ターゲット情報

  • 機能Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
  • 組織特異性Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
  • 配列類似性Belongs to the C5-methyltransferase family.
    Contains 2 BAH domains.
    Contains 1 CXXC-type zinc finger.
  • ドメインThe N-terminal part is required for homodimerization and acts as a regulatory domain.
  • 翻訳後修飾Sumoylated; sumoylation increases activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADCADN antibody
    • AIM antibody
    • CXXC finger protein 9 antibody
    • CXXC-type zinc finger protein 9 antibody
    • CXXC9 antibody
    • DNA (cytosine 5 ) methyltransferase 1 antibody
    • DNA (cytosine-5)-methyltransferase 1 antibody
    • DNA methyltransferase 1 antibody
    • DNA methyltransferase HsaI antibody
    • DNA methyltransferase M.HsaI. antibody
    • DNA MTase antibody
    • DNA MTase HsaI antibody
    • DNMT 1 antibody
    • DNMT antibody
    • Dnmt1 antibody
    • DNMT1_HUMAN antibody
    • Dnmt1o antibody
    • FLJ16293 antibody
    • HSN1E antibody
    • M.HsaI antibody
    • MCMT antibody
    • Met1 antibody
    • MGC104992 antibody
    • mMmul antibody
    • MommeD2 antibody
    see all

Anti-Dnmt1 antibody - ChIP Grade 画像

  • All lanes : Anti-Dnmt1 antibody - ChIP Grade (ab87656) at 1 µg/ml

    Lane 1 : lysate from Mouse erythrolekemia cells MEL11A2
    Lane 2 : lysate from untransfected Mouse myoblast cells C2C12
    Lane 3 : lysate from C2C12 cells transfected with Dnmt1 overexpressing plasmid
    Lane 4 : lysate from C2C12 cells transfected with negative control plasmid


    Predicted band size : 183 kDa
  • Lysate from Mouse erythrolekemia cells MEL11A2 (lane 1), untransfected Mouse myoblast cells C2C12 (lane 2) , C2C12 cells transfected with Dnmt1 overexpressing plasmid (lane 3) and C2C12 cells transfected with negative control plasmid (lane 4) were immunoprecipitated under denaturing conditions using ab87656.
    The immunoprecipitae was subsequently loaded on a gel and probed with ab87656 at 1yg/ml.

Anti-Dnmt1 antibody - ChIP Grade (ab87656) 使用論文

This product has been referenced in:
  • Huang X  et al. Targeted delivery of microRNA-29b by transferrin-conjugated anionic lipopolyplex nanoparticles: a novel therapeutic strategy in acute myeloid leukemia. Clin Cancer Res 19:2355-67 (2013). WB . Read more (PubMed: 23493348) »
  • Chaturvedi CP  et al. Maintenance of gene silencing by the coordinate action of the H3K9 methyltransferase G9a/KMT1C and the H3K4 demethylase Jarid1a/KDM5A. Proc Natl Acad Sci U S A 109:18845-50 (2012). WB ; Mouse . Read more (PubMed: 23112189) »

See all 3 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (mouse embryonic stem cells)
Permeabilization Yes - 0.1% Tween (PBS)
Gating Strategy P1+single-cells
Specification mouse embryonic stem cells
Fixation IC fixation buffer
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Ms. Liliana Antunes

Verified customer

投稿 Nov 30 2016

Thank you for contacting us.

We will guarantee our antibodies for any species and applications listed as tested on our datasheets, even if the reviews are negative. If for any reason you are unsatisfied with your results when using one of our...

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The anti-Dnmt1 antibody ab87656 was used for ChIP with mouse embryonic stem cells. I hope this is helpful. Please contact us again if you have any further questions.

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Mammary gland, liver, mammary gland tumor)
Loading amount 50 µg
Specification Mammary gland, liver, mammary gland tumor
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Jun 17 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"