製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Dnmt1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-RFESPPKTQPTEDN

    , corresponding to internal sequence amino acids 875-888 of human Dnmt1 (NP_001370.1).

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab77689 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報
    Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/32,000.
    WB: Use at a concentration of 0.5 - 1.5 µg/ml. Predicted molecular weight: 183 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
    • 組織特異性
      Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
    • 配列類似性
      Belongs to the C5-methyltransferase family.
      Contains 2 BAH domains.
      Contains 1 CXXC-type zinc finger.
    • ドメイン
      The N-terminal part is required for homodimerization and acts as a regulatory domain.
    • 翻訳後修飾
      Sumoylated; sumoylation increases activity.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ADCADN antibody
      • AIM antibody
      • CXXC finger protein 9 antibody
      • CXXC-type zinc finger protein 9 antibody
      • CXXC9 antibody
      • DNA (cytosine 5 ) methyltransferase 1 antibody
      • DNA (cytosine-5)-methyltransferase 1 antibody
      • DNA methyltransferase 1 antibody
      • DNA methyltransferase HsaI antibody
      • DNA methyltransferase M.HsaI. antibody
      • DNA MTase antibody
      • DNA MTase HsaI antibody
      • DNMT 1 antibody
      • DNMT antibody
      • Dnmt1 antibody
      • DNMT1_HUMAN antibody
      • Dnmt1o antibody
      • FLJ16293 antibody
      • HSN1E antibody
      • M.HsaI antibody
      • MCMT antibody
      • Met1 antibody
      • MGC104992 antibody
      • mMmul antibody
      • MommeD2 antibody
      see all

    画像

    • Anti-Dnmt1 antibody (ab77689) at 0.5 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 183 kDa
      Observed band size : 183 kDa


      Exposure time : 1 hour

    プロトコール

    参考文献

    ab77689 has not yet been referenced specifically in any publications.

    レビューと Q&A

    There are currently no Customer reviews or Questions for ab77689.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録