製品の概要

  • 製品名Anti-DNMBP antibody
    DNMBP 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to DNMBP
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    非交差種: Xenopus laevis
  • 免疫原

    Full length human DNMBP protein (AAH41628.1).

  • ポジティブ・コントロール
    • Hela cell lysate; DNMBP transfected 293T cell lysate Hela cell

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab88534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報ICC/IF: Use at a concentration of 10 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 177 kDa for isoform 1, and 93 kDa for isoform 2.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Scaffold protein that links dynamin with actin-regulating proteins. May play a role in membrane trafficking between the cell surface and the Golgi.
    • 組織特異性Detected in heart, brain, lung, liver, skeletal muscle, kidney and pancreas.
    • 配列類似性Contains 1 BAR domain.
      Contains 1 DH (DBL-homology) domain.
      Contains 6 SH3 domains.
    • 細胞内局在Cytoplasm. Golgi apparatus > Golgi stack. Cytoplasm > cytoskeleton. Cell junction > synapse. Localized to synapses and Golgi stacks.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Dnmbp antibody
      • DNMBP_HUMAN antibody
      • Dynamin binding protein antibody
      • Dynamin-binding protein antibody
      • KIAA1010 antibody
      • Scaffold protein Tuba antibody
      • TUBA antibody
      see all

    Anti-DNMBP antibody 画像

    • Anti-DNMBP antibody (ab88534) at 1 µg/ml + Hela cell lysate at 50 µg

      Predicted band size : 177 , 93 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-DNMBP antibody (ab88534) at 1 µg/ml

      Lane 1 : DNMBP transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 50 µg per lane.


      Predicted band size : 177 , 93 kDa
      Observed band size : 115 kDa (why is the actual band size different from the predicted?)
    • ab88534, at 10µg/ml, staining DNMBP in Hela cell by Immunofluorescence.

    Anti-DNMBP antibody (ab88534) 使用論文

    ab88534 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 500 µg
    Gel Running Conditions Reduced Denaturing
    Sample Xenopus laevis Cell lysate - whole cell (Stage 11 and Stage 32 Embryos)
    Specification Stage 11 and Stage 32 Embryos
    Blocking step KPL Blocking Reagent as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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    投稿 Jun 10 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"