This antibody gave a positive result in ELISA against the immunizing peptide (ab39926).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RNA-binding factor that positively regulates gene expression by prohibiting miRNA-mediated gene suppression. Relieves miRNA repression in germline cells (By similarity). Prohibits the function of several miRNAs by blocking the accessibility of target mRNAs. Sequence-specific RNA-binding factor that binds specifically to U-rich regions (URRs) in the 3' untranslated region (3'-UTR) of several mRNAs. Does not bind to miRNAs. May play a role during primordial germ cell (PGC) survival (By similarity). However, does not seem to be essential for PGC migration.
Contains 2 RRM (RNA recognition motif) domains.
Nucleus. Cytoplasm. Perinuclear germ granules, also called germ plasm or chromatoid body. Colocalizes in perinuclear sites with APOBEC3.
IHC image of Dnd1 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39928, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
has not yet been referenced specifically in any publications.