製品の概要

  • 製品名Anti-DNASE2B antibody
    DNASE2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNASE2B
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: WMAQRLKTHL LTETWQRKRQ ELPSNCSLPY HVYNIKAIKL SRHSYFSSYQ, corresponding to amino acids 254-303 of Human DNASE2B

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 19, 34 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:312500.

ターゲット情報

  • 関連性DNASE2B shares considerable sequence similarity to, and is structurally related to DNase II. The latter is a well characterized endonuclease that catalyzes DNA hydrolysis in the absence of divalent cations at acidic pH. Unlike DNase II which is ubiquitously expressed, expression of this protein is restricted to the salivary gland and lungs. The gene has been localized to chromosome 1p22.3 adjacent (and in opposite orientation) to the uricase pseudogene. Two transcript variants encoding different isoforms have been described for this gene.
  • 細胞内局在Lysosome
  • 参照データベース
  • 別名
    • Deoxyribonuclease 2 beta antibody
    • Deoxyribonuclease 2 beta precursor antibody
    • Deoxyribonuclease II beta antibody
    • DLAD antibody
    • DNase 2 like acid DNase antibody
    • DNASE 2B antibody
    • DNase II beta antibody
    • DNase II like acid DNase antibody
    • DNASE2 antibody
    • DNase2 like acid DNase antibody
    • DnaseIIb antibody
    • EC 3.1.22.1 antibody
    • Endonuclease DLAD antibody
    • Lysosomal DNase II antibody
    • UOX antibody
    see all

Anti-DNASE2B antibody 画像

  • Anti-DNASE2B antibody (ab66738) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 19 kDa,~70 kDa. We are unsure as to the identity of these extra bands.

Anti-DNASE2B antibody (ab66738) 使用論文

ab66738 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66738.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"