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Peptide corresponding to amino acids 347 to 360 of human DNase II precursor.
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNAfragmentation (Zamzami & Kroemer, 1999). The genes encoding human (Yasuda et al.1998, Shiokawa & Tanuma, 1998, Baker et al, 1998), porcine, and murine DNase II have been cloned (Krieser & Eastman, 1998). The DNaseII gene encodes a 40 kDa proenzyme. The mature enzymeconsists of two non-identical subunits, the 32 kDa (a) and 12kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (Shiokawa & Tanuma, 1998). DNase II is ubiquitously expressed in human tissues.
Our Abpromise guarantee covers the use of ab8119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 36 (precursor) kDa).Can be blocked with DNase II peptide (ab8373).|
ab8119 at 5µg/ml staining DNase II in human spleen tissue by IHC