製品の概要

  • 製品名Anti-DNase I antibody
    DNase I 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNase I
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 252 of Human DNase I (NP_005214).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, Raji cell lysates and Molt-4 whole cell lysate. IF/ICC: Caco-2 cell line

法規制情報

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab97393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 31 kDa.

ターゲット情報

  • 機能Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization.
  • 組織特異性Principally in tissues of the digestive system. Highest levels found in urine, but also relatively abundant in semen and saliva.
  • 配列類似性Belongs to the DNase I family.
  • 細胞内局在Secreted. Nucleus envelope. Secretory protein, stored in zymogen granules and found in the nuclear envelope.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Deoxyribonuclease 1 antibody
    • Deoxyribonuclease I antibody
    • Deoxyribonuclease-1 antibody
    • Deoxyribonuclease1 antibody
    • DeoxyribonucleaseI antibody
    • DNAS1_HUMAN antibody
    • DNASE 1 antibody
    • DNase I antibody
    • DNase I lysosomal antibody
    • DNASE1 antibody
    • DNaseI antibody
    • DNL 1 antibody
    • DNL1 antibody
    • Dornase alfa antibody
    • DRNI antibody
    • FLJ38093 antibody
    • Human urine deoxyribonuclease I antibody
    see all

Anti-DNase I antibody 画像

  • ICC/IF image of ab97393 stained Caco-2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97393, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-DNase I antibody (ab97393) at 1/1000 dilution + Molt-4 whole cell lysate at 30 µg

    Predicted band size : 31 kDa
    10% SDS PAGE
  • Anti-DNase I antibody (ab97393) at 1/1000 dilution + Recombinant human DNase I protein (ab73430) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

Anti-DNase I antibody (ab97393) 使用論文

ab97393 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97393.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"