製品の概要

  • 製品名Anti-DNALI1 antibody
    DNALI1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNALI1
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (VTANKAHTGQ GSCWVATLAS AMIPPADSLL KYDTPVLVSR NTEKRSPKAR) of Human DNALI1 (NP_003453).

  • ポジティブ・コントロール
    • Transfected 293T cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab87075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 30 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能May play a dynamic role in flagellar motility.
  • 組織特異性Expressed in many tissues. A smaller 0.9 kb and a larger 2.5 kb transcripts were detected at the highest level in the testis, at medium levels in the prostate, heart, liver, lung and pancreas, at low levels in the ovary, skeletal muscle and small intestine. Not detected in spleen, colon epithelium, thymus or peripheral blood leukocytes. The 0.9 kb transcript is expressed at a 20-fold higher level than the 2.5 kb transcript in the testis.
  • 配列類似性Belongs to the inner dynein arm light chain family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • axonemal antibody
    • Axonemal dynein light chain (hp28) antibody
    • Axonemal dynein light chain antibody
    • Axonemal dynein light intermediate polypeptide 1 antibody
    • dJ423B22.5 antibody
    • DNALI 1 antibody
    • DNALI1 antibody
    • Dynein axonemal light intermediate chain 1 antibody
    • Dynein axonemal light intermediate polypeptide 1 antibody
    • hp28 antibody
    • IDLC_HUMAN antibody
    • Inner dynein arm homolog of clamydomonas antibody
    • Inner dynein arm light chain antibody
    • Inner dynein arm light chain axonemal antibody
    • P28 antibody
    see all

Anti-DNALI1 antibody 画像

  • Species+tissue/cell type:Human nasal epithelial cells. Primary antibody dilution: 1:1000. Secondary antibody: Goat anti-rabbit Alexa Fluor 546. Secondary antibody dilution:1:1000

  • Anti-DNALI1 antibody (ab87075) at 1 µg/ml (in 5% skim milk / PBS buffer) + Transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 30 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%

Anti-DNALI1 antibody (ab87075) 使用論文

This product has been referenced in:
  • Miao C  et al. Mutations in the Motile Cilia Gene DNAAF1 Are Associated with Neural Tube Defects in Humans. G3 (Bethesda) 6:3307-3316 (2016). ICC/IF . Read more (PubMed: 27543293) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"