製品の概要

  • 製品名
    Anti-DnaK antibody [8E2/2]
    DnaK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [8E2/2] to DnaK
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Full length protein (Escherichia coli )

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    8E2/2
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab69617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).

ターゲット情報

  • 関連性
    DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates.
  • 細胞内局在
    Cytoplasm. Cell inner membrane; Peripheral membrane protein.
  • 参照データベース
    • 別名
      • Chaperone Hsp70 antibody
      • Chaperone protein dnaK antibody
      • Co chaperone with DnaJ antibody
      • dnaK antibody
      • Heat shock 70 kDa protein antibody
      • HSP70 antibody
      see all

    画像

    • Anti-DnaK antibody [8E2/2] (ab69617) at 0.1 µg/ml + E.Coli DnaK protein

      Predicted band size : 70 kDa
      Observed band size : 70 kDa

    プロトコール

    参考文献

    This product has been referenced in:
    • Huang Q  et al. A signal sequence suppressor mutant that stabilizes an assembled state of the twin arginine translocase. Proc Natl Acad Sci U S A 114:E1958-E1967 (2017). WB . Read more (PubMed: 28223511) »
    • Elhadad D  et al. Differences in Host Cell Invasion and Salmonella Pathogenicity Island 1 Expression between Salmonella enterica Serovar Paratyphi A and Nontyphoidal S. Typhimurium. Infect Immun 84:1150-65 (2016). Read more (PubMed: 26857569) »

    See all 10 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Sample
    Sodalis glossinidius Cell lysate - whole cell (bacteria)
    Loading amount
    30 µg
    Specification
    bacteria
    Treatment
    42C heatshock for 5 min
    Gel Running Conditions
    Reduced Denaturing (12.5% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Laura Runyen-Janecky

    Verified customer

    投稿 Mar 01 2013

    DISCOUNT CODE: ****
    Expiration date: March 27, 2013
    Value: $337

    We do not have data for that species but I am very pleased to hear you would like to accept our offer and test ab69617 on samples of Sodalis glossinidius. This code wi...

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    Thank you for your reply.

    You are correct that you have to purchase the antibody in question first (at full price, but after you have submitted the Abreview on the antibody performed (and it can be positive or negative), you can use the code ...

    Read More

    Thank you for contacting Abcam
    .
    To our knowledge, ab69617 has not been tested in with other bacterial species, including the Sodlalis DnaK protein. However by participating in our AbTrial program you can now use our products in an untested a...

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    DISCOUNT CODE: ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test ab69617 in Xanthomonas campestris pv. Campestris. The homology between e. coli DnaK and Xcc DnaK is 72.6% (see attached pdf fo...

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    Thank you and your customer for providing the sequence of DnaK from Xanthomonas campestris pv. Campestris. Both antibodies were generated with the full-length DnaK protein; ab69617 with E.coli DnaK and ab73473 with DnaK from Mycobacterium avium ssp....

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    Thank you for your inquiry. Unfortunately, I do not have access to the sequence of DnaK of Xanthomonas campestris pv. Campestris. Could you please ask your customer to provide me with this sequence? I will then make an alignment. If the homolo...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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