製品の概要

  • 製品名Anti-DnaK antibody [7D9D7]
    DnaK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7D9D7] to DnaK
  • アプリケーション適用あり: IHC-P, Electron Microscopy, ELISA, WBmore details
  • 種交差性
    Reacts with mycobacterial HSP70 and cross-reactive with E. coli DnaK.
  • 免疫原

    DnaK from Mycobacterium avium ssp. paratuberculosis (MAP).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名7D9D7
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab73473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
Electron Microscopy Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.

ターゲット情報

  • 関連性DnaK is the prokaryotic analogue of eukaryotic Hsp70. Heat shock proteins applies to a group of proteins that assist in the assembly, folding, and translocation of other proteins. In addition, they protect the cell against heat injury or other forms of stress. All cells, prokaryotic and eukaryotic, are able to respond to different cellular stresses by synthesizing these proteins. Heat shock proteins are highly conserved, ubiquitously distributed, and involved in important aspects of viral and bacterial infections, autoimmune diseases, and in cancer immunity. Two families of molecular chaperones have been identified. The members of the Hsp70 family (DnaK/DnaJ/GrpE) bind to the growing polypeptide chain and prevent its premature folding. The chaperonin family (GroEL and GroES) assists in correct folding when the complete polypeptide chain is formed and is transported into the cytosol or mitochondria. All the major heat shock proteins help to suppress irreversible unfolding reactions. These protein folding 'assistants' may have important functions in amyloid diseases where incorrectly folded proteins accumulate as folded aggregates.
  • 細胞内局在Cytoplasm. Cell inner membrane; Peripheral membrane protein.
  • 別名
    • Chaperone Hsp70 antibody
    • Chaperone protein dnaK antibody
    • Co chaperone with DnaJ antibody
    • dnaK antibody
    • Heat shock 70 kDa protein antibody
    • HSP70 antibody
    see all

Anti-DnaK antibody [7D9D7] (ab73473) 使用論文

This product has been referenced in:
  • Santema W  et al. Hsp70 vaccination-induced antibodies recognize B cell epitopes in the cell wall of Mycobacterium avium subspecies paratuberculosis. Vaccine : (2010). Read more (PubMed: 21199702) »
  • Burt SA  et al. Carvacrol induces heat shock protein 60 and inhibits synthesis of flagellin in Escherichia coli O157:H7. Appl Environ Microbiol 73:4484-90 (2007). Read more (PubMed: 17526792) »

See all 3 Publications for this product

Product Wall

Thank you and your customer for providing the sequence of DnaK from Xanthomonas campestris pv. Campestris. Both antibodies were generated with the full-length DnaK protein; ab69617 with E.coli DnaK and ab73473 with DnaK from Mycobacterium avium ssp....

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Thank you for your inquiry. Unfortunately, I do not have access to the sequence of DnaK of Xanthomonas campestris pv. Campestris. Could you please ask your customer to provide me with this sequence? I will then make an alignment. If the homolo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"