Anti-DNAJC3 抗体 [EP597Y] (ab52899)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP597Y] to DNAJC3
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-DNAJC3 antibody [EP597Y]
DNAJC3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP597Y] to DNAJC3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human DNAJC3 aa 1-100 (N terminal). The exact sequence is proprietary.
-
ポジティブ・コントロール
- HeLa cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP597Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52899の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
-
機能
Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity. -
組織特異性
Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels. -
配列類似性
Contains 1 J domain.
Contains 9 TPR repeats. -
ドメイン
The J domain mediates interaction with HSPA8. -
細胞内局在
Endoplasmic reticulum. - Information by UniProt
-
参照データベース
- Entrez Gene: 5611 Human
- Omim: 601184 Human
- SwissProt: Q13217 Human
- Unigene: 59214 Human
-
別名
- DnaJ (Hsp40) homolog subfamily C member 3 antibody
- DnaJ homolog subfamily C member 3 antibody
- DNAJC3 antibody
see all
画像
-
Anti-DNAJC3 antibody [EP597Y] (ab52899) at 1/1000 dilution + HeLa cell lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 58 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (1)
ab52899 は 1 報の論文で使用されています。
- Bergmann L et al. Subcellular Localization and Mitotic Interactome Analyses Identify SIRT4 as a Centrosomally Localized and Microtubule Associated Protein. Cells 9:N/A (2020). PubMed: 32846968