製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DNAJC3
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human DNAJC3.

    (Peptide available as ab72884.)

  • ポジティブ・コントロール
    • This antibody gives a positive signal in the following lysates: Rat Thymus Tissue TE 671 Whole Cell Y79 Whole Cell Hela - Staurosporine Treated (24hr, 500nM) Jurkat - Staurosporine Treated (24hr, 500nM).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 機能
    Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
  • 組織特異性
    Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
  • 配列類似性
    Contains 1 J domain.
    Contains 9 TPR repeats.
  • ドメイン
    The J domain mediates interaction with HSPA8.
  • 細胞内局在
    Endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DnaJ (Hsp40) homolog subfamily C member 3 antibody
    • DnaJ homolog subfamily C member 3 antibody
    • DNAJC3 antibody
    • DNJC3_HUMAN antibody
    • double-stranded RNA-activated protein kinase inhibitor antibody
    • Endoplasmic reticulum DnaJ protein 6 antibody
    • ERdj6 antibody
    • HP58 antibody
    • Interferon induced, double stranded RNA activated protein kinase inhibitor antibody
    • Interferon-induced antibody
    • P58 antibody
    • p58 ipk antibody
    • P58IPK antibody
    • PRKRI antibody
    • Protein kinase inhibitor of 58 kDa antibody
    • Protein kinase inhibitor p58 antibody
    • Protein kinase interferon inducible double stranded RNA dependent inhibitor antibody
    see all

画像

  • All lanes : Anti-DNAJC3 antibody (ab72885) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
    Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 5 : Thymus (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 58 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab72885 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72885, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HepG2, Hek293, MCF-7 cells at 1µg/ml.

参考文献

ab72885 has not yet been referenced specifically in any publications.

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