製品の概要
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製品名Anti-DNAJC21 antibody
DNAJC21 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to DNAJC21
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アプリケーション適用あり: WB, IPmore details
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種交差性交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)
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ポジティブ･コントロール
- Whole cell lysate from HeLa and 293T cells.
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特記事項Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab86434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| IP |
追加情報
IP: Use at 2 - 5µg/mg of lysate.
WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能May act as a co-chaperone for HSP70.
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組織特異性Brain, placenta, kidney and pancreas.
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配列類似性Contains 2 C2H2-type zinc fingers.
Contains 1 J domain. - Information by UniProt
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参照データベース
- Entrez Gene: 134218 Human
- SwissProt: Q5F1R6 Human
- Unigene: 131887 Human
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別名
- DJC21_HUMAN antibody
- DnaJ homolog subfamily A member 5 antibody
- DnaJ homolog subfamily C member 21 antibody
see all
Anti-DNAJC21 antibody 画像
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Western blot - DNAJC21 antibody (ab86434)All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml
Lane 1 : HeLa cell lysate at 50 µg
Lane 2 : HeLa cell lysate at 15 µg
Lane 3 : HeLa cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?) -
Immunoprecipitation - DNAJC21 antibody (ab86434)Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.
Anti-DNAJC21 antibody (ab86434) 使用論文
This product has been referenced in:
- Tummala H et al. DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation. Am J Hum Genet 99:115-24 (2016). Read more (PubMed: 27346687) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"