製品の概要

  • 製品名
    Anti-DNAJC21 antibody
    DNAJC21 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNAJC21
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 400-450 of Human DNAJC21 (NP_001012339.2)

  • ポジティブ･コントロール
    • Whole cell lysate from HeLa and 293T cells.
  • 特記事項
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報
    IP: Use at 2 - 5µg/mg of lysate.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能
      May act as a co-chaperone for HSP70.
    • 組織特異性
      Brain, placenta, kidney and pancreas.
    • 配列類似性
      Contains 2 C2H2-type zinc fingers.
      Contains 1 J domain.
    • Information by UniProt
    • 参照データベース
      • 別名
        • DJC21_HUMAN antibody
        • DnaJ homolog subfamily A member 5 antibody
        • DnaJ homolog subfamily C member 21 antibody
        • DNAJA5 antibody
        • dnajc21 antibody
        • GS3 antibody
        • JJJ1 antibody
        • Protein GS3 antibody
        see all

      画像

      • Western blot - Anti-DNAJC21 antibody (ab86434)
        Western blot - Anti-DNAJC21 antibody (ab86434)
        All lanes : Anti-DNAJC21 antibody (ab86434) at 0.04 µg/ml

        Lane 1 : HeLa cell lysate at 50 µg
        Lane 2 : HeLa cell lysate at 15 µg
        Lane 3 : HeLa cell lysate at 5 µg
        Lane 4 : 293T cell lysate at 50 µg

        Predicted band size: 62 kDa
        Observed band size: 70 kDa (why is the actual band size different from the predicted?)

      • Immunoprecipitation - Anti-DNAJC21 antibody (ab86434)
        Immunoprecipitation - Anti-DNAJC21 antibody (ab86434)
        Detection of Human DNAJC21 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86434 at 3 ug/mg lysate. Subsequent Western blot analysis was performed using ab86434 at 1 ug/ml.

      参考文献

      This product has been referenced in:
      • Tummala H  et al. DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation. Am J Hum Genet 99:115-24 (2016). Read more (PubMed: 27346687) »

      See 1 Publication for this product

      ab86434 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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