Recombinant Fragment 交差が予測される動物種:
Mouse, Rat, Horse, Chicken, Cow, Dog, Human, Chimpanzee, Rhesus monkey
Recombinant fragment: LDPHQKDDIN KKAFDKFKKE HGVVPQADNA TPSERFEGPY TDFTPWTTEE QKLLEQALKT YPVNTPERWE KIAEAVPGRT KKDCMKRYKE LVEMVKAKKA A, corresponding to amino acids 408-509 of Human DNAJC2 (XP_168590) with tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 72 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
配列類似性Contains 1 J domain. Contains 2 SANT domains.
ドメインThe ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.