製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to DNAJB9
  • アプリケーション
    適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    RGNMVTTYTDCSGQ

    , corresponding to C terminal amino acids 210-223 of Human MDG1.

  • ポジティブ・コントロール
    • Human kidney
  • 特記事項
    GenBank Accession Number – NP_036460.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/4000. This antibody gave a positive result in ELISA against the immunizing peptide.
WB Use at an assay dependent dilution. Predicted molecular weight: 26 kDa. Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1µg/ml.
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

画像

  • ab6053 at 4 µg/ml staining MDG1 in Human kidney by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Detected with HRP-staining.

参考文献

ab6053 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us for technical support. I'm sorry to hear that ab6053 is not working well for you. This antibody is a FastTrack product and as such has not been fully characterised yet by our laboratory as we do not have good positive con...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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