製品の概要

  • 製品名Anti-DNAJB2 antibody
    DNAJB2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DNAJB2
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human, Recombinant Fragment
  • 免疫原

    Recombinant fragment: LELSRREQQP SVTSRSGGTQ VQQTPASCPL DSDLSEDEDL QLAMAYSLSE MEAAGKKPAG GREAQHRRQG RPKAQHQDPG LGGTQEGARG EATKRSPSPE EKASRCLIL, corresponding to amino acids 216-324 of Human DNAJB2 (NP_006727) with a tag.

  • ポジティブ・コントロール
    • A431 cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA Use at an assay dependent dilution. Detection limit is approximately 0.3ng/ml as a capture antibody when used against the immunogen.

ターゲット情報

  • 関連性DNAJB2 is involved in heat shock protein and unfolded protein binding.
  • 参照データベース
  • 別名
    • Descriptions antibody
    • DnaJ (Hsp40) homolog subfamily B member 2 antibody
    • DnaJ homolog subfamily B member 2 antibody
    • DnaJ protein homolog 1 antibody
    • DNAJB2 antibody
    • Heat shock 40 kDa protein 3 antibody
    • Heat shock protein J1 antibody
    • Heat shock protein neuronal DNAJ like 1 antibody
    • HSJ 1 antibody
    • HSJ1 antibody
    • HSPF3 antibody
    see all

Anti-DNAJB2 antibody 画像

  • Anti-DNAJB2 antibody (ab77322) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • Anti-DNAJB2 antibody (ab77322) at 5 µg/ml + A431 cell lysate at 50 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
  • ab77322 was used as a capture antibody and detection limit for recombinant tagged DNAJB2 is approximately 0.3ng/ml.

Anti-DNAJB2 antibody (ab77322) 使用論文

ab77322 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab77322.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"