製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DNAJB11
  • 特異性
    ab75107 detects endogenous levels of total DNAJB11 protein.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from an internal region of human DNAJB11.

  • ポジティブ・コントロール
    • IHC-P: human heart tissue. WB: extracts from HeLa cells, HUVEC cells, COLO cells and MCF-7 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75107 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Serves as a co-chaperone for HSPA5. Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains, but dissociates from the HSPA5-unfolded protein complex before folding is completed. May help recruiting HSPA5 and other chaperones to the substrate. Stimulates HSPA5 ATPase activity.
  • 組織特異性
    Widely expressed.
  • 配列類似性
    Contains 1 J domain.
  • 翻訳後修飾
    Contains high-mannose Endo H-sensitive carbohydrates.
    Cys-169, Cys-171, Cys-193 and Cys-196 form intramolecular disulfide bonds. The preferential partner for each Cys is not known.
    Thr-188 was reported (PubMed:17525332) to be phosphorylated upon DNA damage by ATM or ATR; however as this position has been shown to be in the ER lumen, the in vivo relevance is not proven.
  • 細胞内局在
    Endoplasmic reticulum lumen. Associated with the ER membrane in a C-terminally epitope-tagged construct.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ABBP-2 antibody
    • ABBP2 antibody
    • APOBEC1 binding protein 2 antibody
    • APOBEC1-binding protein 2 antibody
    • DJ9 antibody
    • DJB11_HUMAN antibody
    • DnaJ (Hsp40) homolog subfamily B member 11 antibody
    • DnaJ homolog subfamily B member 11 antibody
    • DnaJ protein homolog 9 antibody
    • DNAJB11 antibody
    • EDJ antibody
    • ER associated DNAJ antibody
    • ER associated dnaJ protein 3 antibody
    • ER associated Hsp40 co chaperone antibody
    • ER-associated DNAJ antibody
    • ER-associated dnaJ protein 3 antibody
    • ER-associated Hsp40 co-chaperone antibody
    • ERdj3 antibody
    • ERJ3 antibody
    • ERj3p antibody
    • hDj-9 antibody
    • hDj9 antibody
    • HEDJ antibody
    • Human DnaJ protein 9 antibody
    • PWP1 interacting protein 4 antibody
    • PWP1-interacting protein 4 antibody
    see all

画像

  • All lanes : Anti-DNAJB11 antibody (ab75107) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from HUVEC cells
    Lane 3 : extracts from COLO cells
    Lane 4 : extracts from MCF-7 cells
    Lane 5 : extracts from HeLa cells with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Immunohistochemical analysis of DNAJB11 in paraffin embedded human heart tissue using 1/50 ab75107.
    Left image: untreated sample.
    Right image: sample treated with immunising peptide.

参考文献

This product has been referenced in:
  • Croner RS  et al. Quantitative proteome profiling of lymph node-positive vs. -negative colorectal carcinomas pinpoints MX1 as a marker for lymph node metastasis. Int J Cancer 135:2878-86 (2014). Read more (PubMed: 24771638) »
  • Xu W  et al. Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis. Biol Reprod 89:142 (2013). Read more (PubMed: 24198121) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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