製品の概要

  • 製品名Anti-DNAH6 antibody
  • 製品の詳細
    Rabbit polyclonal to DNAH6
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    TKLNTILCQT FVFCYLWSLG GNLTENYYDS FDTFIRTQFD DNPDARLPNS GDLWSIHMDF DTKRLDPWER IIPTFKYNRD VP internal sequence amino acids 1983-2064 of Human DNAH6 (Q9C0G6).

  • ポジティブ・コントロール
    • Human fallopian tube tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/850. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP.
  • 組織特異性Detected in brain, testis and trachea.
  • 配列類似性Belongs to the dynein heavy chain family.
  • ドメインDynein heavy chains probably consist of an N-terminal stem (which binds cargo and interacts with other dynein components), and the head or motor domain. The motor contains six tandemly-linked AAA domains in the head, which form a ring. A stalk-like structure (formed by two of the coiled coil domains) protrudes between AAA 4 and AAA 5 and terminates in a microtubule-binding site. A seventh domain may also contribute to this ring; it is not clear whether the N-terminus or the C-terminus forms this extra domain. There are four well-conserved and two non-conserved ATPase sites, one per AAA domain. Probably only one of these (within AAA 1) actually hydrolyzes ATP, the others may serve a regulatory function.
  • 細胞内局在Cytoplasm > cytoskeleton > cilium axoneme.
  • Information by UniProt
  • 参照データベース
  • 別名
    • axonemal antibody
    • Axonemal beta dynein heavy chain 6 antibody
    • Ciliary dynein heavy chain 6 antibody
    • DNAH6 antibody
    • Dnahc6 antibody
    • DNHL1 antibody
    • DYH6_HUMAN antibody
    • Dynein heavy chain 6 antibody
    • FLJ37357 antibody
    • HL-2 antibody
    see all

Anti-DNAH6 antibody 画像

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm. Recommended concentration of ab122333 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122333, at a 1/850 dilution, staining DNAH6 in glandular cells of paraffin-embedded Human fallopian tube tissue by immunohistochemistry.

Anti-DNAH6 antibody (ab122333) 使用論文

ab122333 has not yet been referenced specifically in any publications.

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