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Protein-DNA interactions play a critical role for cellular functions such as signal transduction, gene transcription and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanishm of protein-DNA interaction is important for understanding cellular processes.
DNA-Protein Binding Assay Kit (Fluorometric) (ab117140) allows the user to investigate direct in vitro protein-DNA interaction, using dsDNA of choice, within 3 hours. ab117139 is specifically suitable for detection of transcription factor activation in mammalian tissue and cell extracts.
|10X Wash Buffer||1 x 20ml|
|8-Well Assay Strips (with Frame)||12 units|
|96-Well Assay Plate||1 unit|
|Antibody Buffer||1 x 6ml|
|Assay Buffer||1 x 10ml|
|DTT (500X)||1 x 22µl|
|Extract Cleaner||1 x 100µl|
|Fluoro Developer||1 x 0.2ml|
|Oligo Release Solution||1 x 11ml|
Our Abpromise guarantee covers the use of ab117140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117140 has not yet been referenced specifically in any publications.