製品の概要

  • 製品名Anti-DNA Polymerase theta antibody
    DNA Polymerase theta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Polymerase theta
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: SFTSSDDIAE VLFLELKLPP NREMKNQGSK KTLGSTRRGI DNGRKLRLGR , corresponding to C terminal amino acids 2125-2174 of Human DNA Polymerase theta

  • ポジティブ・コントロール
    • Placenta lysate IF/ICC: HepG2 cell line

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 198 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/12500.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.
  • 組織特異性Highly expressed in testis.
  • 配列類似性Belongs to the DNA polymerase type-A family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781A0112 antibody
    • DNA polymerase eta antibody
    • DNA polymerase theta antibody
    • DPOLQ_HUMAN antibody
    • POLH antibody
    • POLQ antibody
    • Polymerase (DNA directed) theta antibody
    • PRO0327 antibody
    see all

Anti-DNA Polymerase theta antibody 画像

  • Anti-DNA Polymerase theta antibody (ab80906) at 1 µg/ml + placenta lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 198 kDa
    Observed band size : 160 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab80906 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80906, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DNA Polymerase theta antibody (ab80906) 使用論文

This product has been referenced in:
  • Ceccaldi R  et al. Homologous-recombination-deficient tumours are dependent on Pol?-mediated repair. Nature 518:258-62 (2015). Read more (PubMed: 25642963) »
  • Yoon JH  et al. A role for DNA polymerase ? in promoting replication through oxidative DNA lesion, thymine glycol, in human cells. J Biol Chem 289:13177-85 (2014). WB ; Human . Read more (PubMed: 24648516) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Xenopus laevis Cell lysate - whole cell (X. laevis egg extract)
Specification X. laevis egg extract
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
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Dr. Nikolaos Parisis

Verified customer

投稿 Jul 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"