製品の概要

  • 製品名Anti-DNA Polymerase theta antibody
    DNA Polymerase theta 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DNA Polymerase theta
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Recombinant Fragment
    交差が予測される動物種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1609-1717 of Human DNA Polymerase theta

  • ポジティブ・コントロール
    • Recombinant tagged human DNA Polymerase theta fragment (the immunogen).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 198 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged DNA Polymerase theta is approximately 1 ng/ml when used as a capture antibody.

ターゲット情報

  • 機能Has a DNA polymerase activity on nicked double-stranded DNA and on a singly primed DNA template. The enzyme activity is resistant to aphidicolin, and inhibited by dideoxynucleotides. Exhibites a single-stranded DNA-dependent ATPase activity. Could be involved in the repair of interstrand cross-links.
  • 組織特異性Highly expressed in testis.
  • 配列類似性Belongs to the DNA polymerase type-A family.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp781A0112 antibody
    • DNA polymerase eta antibody
    • DNA polymerase theta antibody
    • DPOLQ_HUMAN antibody
    • POLH antibody
    • POLQ antibody
    • Polymerase (DNA directed) theta antibody
    • PRO0327 antibody
    see all

Anti-DNA Polymerase theta antibody 画像

  • Anti-DNA Polymerase theta antibody (ab77151) at 5 µg/ml + recombinant tagged human DNA Polymerase theta fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 198 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-DNA Polymerase theta antibody (ab77151) 使用論文

ab77151 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab77151.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"