製品の概要

  • 製品名Anti-DNA Polymerase lambda antibody
    DNA Polymerase lambda 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to DNA Polymerase lambda
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide: LGLPYREPAERDW, corresponding to C terminal amino acids 563-571 of Human DNA Polymerase lambda.

  • 特記事項GenBank Accession Number – NP_037406

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
WB
  • 追加情報IHC-P: Use at a concentration of 5 µg/ml.
    Peptide ELISA: antibody detection limit dilution >1/32000.
    Western Blot: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1µg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
    • 組織特異性Expressed in a number of tissues. Abundant in testis.
    • 配列類似性Belongs to the DNA polymerase type-X family.
      Contains 1 BRCT domain.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BETA N antibody
      • BETAN antibody
      • DNA directed DNA polymerase lambda antibody
      • DNA polymerase beta 2 antibody
      • DNA polymerase beta-2 antibody
      • DNA polymerase kappa antibody
      • DNA polymerase kappa DNA polymerase beta N antibody
      • DNA polymerase lambda antibody
      • DNA polymerase lamda2 antibody
      • DPOLL_HUMAN antibody
      • EC 2.7.7.7,EC 4.2.99. antibody
      • FLJ46002 antibody
      • OTTHUMP00000020321 antibody
      • OTTHUMP00000020323 antibody
      • OTTHUMP00000059179 antibody
      • Pol beta2 antibody
      • POL KAPPA antibody
      • Pol Lambda antibody
      • POLKAPPA antibody
      • POLL antibody
      • Polymerase DNA directed lambda antibody
      see all

    Anti-DNA Polymerase lambda antibody 画像

    • ab5954 at 5ug/ml staining DNA Polymerase lambda in human small intestine tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). A AP-staining procedure was used for detection.

    Anti-DNA Polymerase lambda antibody (ab5954) 使用論文

    This product has been referenced in:
    • Selak N  et al. The Bloom's syndrome helicase (BLM) interacts physically and functionally with p12, the smallest subunit of human DNA polymerase delta. Nucleic Acids Res 36:5166-79 (2008). WB ; Human . Read more (PubMed: 18682526) »

    See 1 Publication for this product

    Product Wall

    It shouldn't recognize polymerase beta. By BLAST analysis of the peptide we wouldn't predict any. But this has not been tested experimentally.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"