Anti-DNA Polymerase Kappa/POLK 抗体 (ab86076)

製品の概要

  • 製品名
    Anti-DNA Polymerase Kappa/POLK antibody
    DNA Polymerase Kappa/POLK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Polymerase Kappa/POLK
  • アプリケーション
    適用あり: WBmore details
    適用なし: IP
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 675 and 725 of Human DNA Polymerase Kappa/POLK (NP_057302.1)

  • ポジティブ・コントロール
    • HeLa or 293T whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 99 kDa.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
    • 組織特異性
      Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
    • 配列類似性
      Belongs to the DNA polymerase type-Y family.
      Contains 2 UBZ-type zinc fingers.
      Contains 1 umuC domain.
    • ドメイン
      The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
    • 細胞内局在
      Nucleus. Detected throughout the nucleus and at replication foci.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DinB homolog 1 (E. coli) antibody
      • DINB protein antibody
      • DINB1 antibody
      • DINP antibody
      • DNA damage inducible protein b antibody
      • DNA polymerase kappa antibody
      • Polk antibody
      • POLK_HUMAN antibody
      • POLQ antibody
      • Polymerase (DNA directed) kappa antibody
      • polymerase, DNA, kappa antibody
      see all

    画像

    • All lanes : Anti-DNA Polymerase Kappa/POLK antibody (ab86076) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 99 kDa

    プロトコール

    参考文献

    ab86076 has not yet been referenced specifically in any publications.

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