製品の概要

  • 製品名
    Anti-DNA Polymerase Kappa/POLK antibody
    DNA Polymerase Kappa/POLK 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DNA Polymerase Kappa/POLK
  • アプリケーション
    適用あり: WB, IHC-P, Sandwich ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (proprietary-tag) corresponding to Human DNA Polymerase Kappa/POLK aa 1-472.
    Sequence:

    MDSTKEKCDSYKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFY GNELKKEKQVNQRIENMMQQKAQITSQQLRKAQLQVDRFAMELEQSRNLS NTIVHIDMDAFYAAVEMRDNPELKDKPIAVGSMSMLSTSNYHARRFGVRA AMPGFIAKRLCPQLIIVPPNFDKYRAVSKEVKEILADYDPNFMAMSLDEA YLNITKHLEERQNWPEDKRRYFIKMGSSVENDNPGKEVNKLSEHERSISP LLFEESPSDVQPPGDPFQVNFEEQNNPQILQNSVVFGTSAQEVVKEIRFR IEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPNRQAVMDFIKDLP IRKVSGIGKVTEKMLKALGIITCTELYQQRALLSLLFSETSWHYFLHISL GLGSTHLTRDGERKSMSVERTFSEINKAEEQYSLCQELCSELAQDLQKER LKVLYFDMVSLVFKFFNSKMLP


    Database link: AAH50718

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab115625 as a replacement.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab57070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 99 kDa.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use at an assay dependent concentration.

Detection limit for recombinant tagged DNA Polymerase Kappa/POLK is approximately 0.3 ng/ml as a capture antibody

ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能
    DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
  • 組織特異性
    Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
  • 配列類似性
    Belongs to the DNA polymerase type-Y family.
    Contains 2 UBZ-type zinc fingers.
    Contains 1 umuC domain.
  • ドメイン
    The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • 細胞内局在
    Nucleus. Detected throughout the nucleus and at replication foci.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DinB homolog 1 (E. coli) antibody
    • DINB protein antibody
    • DINB1 antibody
    • DINP antibody
    • DNA damage inducible protein b antibody
    • DNA polymerase kappa antibody
    • Polk antibody
    • POLK_HUMAN antibody
    • POLQ antibody
    • Polymerase (DNA directed) kappa antibody
    • polymerase, DNA, kappa antibody
    see all

画像

  • Anti-DNA Polymerase Kappa/POLK antibody (ab57070) at 1 µg + HeLa nuclear lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG at 1/5000 dilution

    Predicted band size : 99 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue, staining DNA Polymerase Kappa/POLK with ab57070 at 3 µg/ml.

  • Immunofluorescence of HeLa cells, staining DNA Polymerase Kappa/POLK with ab57070 at 10 µg/ml.

  • All lanes : Anti-DNA Polymerase Kappa/POLK antibody (ab57070) at 5 µg/ml

    Lane 1 : DNA Polymerase Kappa/POLK-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    Goat Anti-Mouse IgG
    Developed using the ECL technique

    Predicted band size : 99 kDa
    Additional bands at : 54.1 kDa (possible isoform).
  • Detection limit for recombinant tagged DNA Polymerase Kappa/POLK is approximately 0.3 ng/mL as a capture antibody

参考文献

This product has been referenced in:
  • Guo J  et al. miR-20b downregulates polymerases ? and ? in XP-V tumor cells. Oncol Lett 11:3790-3794 (2016). Read more (PubMed: 27313696) »
  • Ziv O  et al. Identification of novel DNA-damage tolerance genes reveals regulation of translesion DNA synthesis by nucleophosmin. Nat Commun 5:5437 (2014). WB . Read more (PubMed: 25421715) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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