Anti-DNA Polymerase Kappa/POLK 抗体 (ab115625)

製品の概要

  • 製品名Anti-DNA Polymerase Kappa/POLK antibody
    DNA Polymerase Kappa/POLK 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to DNA Polymerase Kappa/POLK
  • アプリケーション適用あり: ELISA, WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    PVNQPKESSRSTGS

    corresponding to amino acids 817-830 of Human DNA Polymerase Kappa/POLK, conjugated to KLH.

  • ポジティブ・コントロール
    • Human prostate tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab115625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/2000 - 1/10000.
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent dilution.

ターゲット情報

  • 機能DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.
  • 組織特異性Detected at low levels in testis, spleen, prostate and ovary. Detected at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
  • 配列類似性Belongs to the DNA polymerase type-Y family.
    Contains 2 UBZ-type zinc fingers.
    Contains 1 umuC domain.
  • ドメインThe catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • 細胞内局在Nucleus. Detected throughout the nucleus and at replication foci.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DinB homolog 1 (E. coli) antibody
    • DINB protein antibody
    • DINB1 antibody
    • DINP antibody
    • DNA damage inducible protein b antibody
    • DNA polymerase kappa antibody
    • Polk antibody
    • POLK_HUMAN antibody
    • POLQ antibody
    • Polymerase (DNA directed) kappa antibody
    • polymerase, DNA, kappa antibody
    see all

Anti-DNA Polymerase Kappa/POLK antibody 画像

  • ab115625, at 5 µg/ml, staining DNA Polymerase Kappa/POLK in formalin fixed, paraffin embedded Human prostate tissue by Immunohistochemistry. Following primary incubation slides were incubated with biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-DNA Polymerase Kappa/POLK antibody (ab115625) 使用論文

ab115625 has not yet been referenced specifically in any publications.

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