製品の概要

  • 製品名
    Anti-DNA Polymerase iota antibody
    DNA Polymerase iota 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Polymerase iota
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 647-676 of Human DNA Polymerase iota (NP_009126.2).

  • ポジティブ・コントロール
    • HL60 cell line lysates.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab123331 was purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab123331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 83 kDa.

ターゲット情報

  • 機能
    Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • 組織特異性
    Ubiquitous. Highly expressed in testis.
  • 配列類似性
    Belongs to the DNA polymerase type-Y family.
    Contains 1 umuC domain.
  • ドメイン
    The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity.
  • 細胞内局在
    Nucleus. Accumulates at replication forks after DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA polymerase iota antibody
    • Eta 2 antibody
    • Eta2 antibody
    • POLI antibody
    • POLI_HUMAN antibody
    • Polymerase (DNA directed) iota antibody
    • RAD 30B antibody
    • RAD30 homolog B antibody
    • RAD30, S. cerevisiae, homolog of antibody
    • RAD30B antibody
    • RAD3OB antibody
    see all

画像

  • Anti-DNA Polymerase iota antibody (ab123331) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 83 kDa

プロトコール

参考文献

ab123331 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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