Anti-DNA Polymerase epsilon 抗体 (ab83442)

製品の概要

  • 製品名Anti-DNA Polymerase epsilon antibody
    DNA Polymerase epsilon 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Polymerase epsilon
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 1-50 (AERPEDLNLPNAVITRIIKEALPDGVNISKEARSAISRAASVFVLYATS C) of Human POLE3 (NP_059139).

  • ポジティブ・コントロール
    • Human fetal lung lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83442 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.Can be blocked with DNA Polymerase epsilon peptide (ab135296). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titer in peptide based assay is 1/1562500.

ターゲット情報

  • 機能Forms a complex with DNA polymerase epsilon subunit CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1.
  • 組織特異性Expressed in all tissues tested, including, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arsenic transactivated protein antibody
    • Arsenic-transactivated protein antibody
    • ASTP antibody
    • CHARAC 17 antibody
    • CHARAC17 antibody
    • CHRAC 17 antibody
    • CHRAC-17 antibody
    • CHRAC17 antibody
    • Chromatin Accessibility Complex 17 antibody
    • Chromatin accessibility complex 17 kDa protein antibody
    • DNA Polymerase Epsilon p17 Subunit antibody
    • DNA polymerase epsilon subunit 3 antibody
    • DNA polymerase epsilon subunit p17 antibody
    • DNA polymerase II subunit 3 antibody
    • DPOE3_HUMAN antibody
    • Histone fold protein CHRAC17 antibody
    • HuCHRAC 17 antibody
    • HuCHRAC17 antibody
    • p17 antibody
    • p17 subunit antibody
    • POL E3 antibody
    • POLE 3 antibody
    • POLE3 antibody
    • Polymerase (DNA directed) epsilon 3 antibody
    • YBL 1 antibody
    • YBL1 antibody
    see all

Anti-DNA Polymerase epsilon antibody 画像

  • Anti-DNA Polymerase epsilon antibody (ab83442) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody.

    Predicted band size : 16 kDa

Anti-DNA Polymerase epsilon antibody (ab83442) 使用論文

ab83442 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam.
We have now been able to add the peptide used to generate ab83442 to the catalogue.
It has been added as: DNA Polymerase epsilon peptide (ab135296)
If there is anything else I can help you with, please let m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"