Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma (left) and mouse teratoma (right) tissues labelling DNA Polymerase epsilon with ab74308 at 1/5000 (0.2µg/ml). Detection: DAB.
Western blot - DNA Polymerase epsilon antibody (ab74308)
All lanes : Anti-DNA Polymerase epsilon antibody (ab74308) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg Lane 2 : Whole cell lysate from HeLa cells at 15 µg Lane 3 : Whole cell lysate from HeLa cells at 5 µg Lane 4 : Whole cell lysate from 293T cells at 50 µg
Immunoprecipitation - DNA Polymerase epsilon antibody (ab74308)
Detection of Human DNA polymerase epsilon by immunoprecipitation of 1mg HeLa cells whole lysate, using ab74308 at 3µg/mg lysate (lane 1, 20% IP loaded) or a control IgG (lane 2, 20% IP loaded). The post-IP WB was performed using ab74308 at 1 µg/ml. Detection: chemiluminescence with an exposure time of 30 seconds.