This antibody gave a positive signal in the following Human tissue lysates: Heart; Brain; Placenta; Testis; Skeletal muscle.
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Chromatin accessibility complex 17 kDa protein antibody
DNA Polymerase Epsilon p17 Subunit antibody
DNA polymerase epsilon subunit 3 antibody
DNA polymerase epsilon subunit p17 antibody
DNA polymerase II subunit 3 antibody
Histone fold protein CHRAC17 antibody
HuCHRAC 17 antibody
p17 subunit antibody
POL E3 antibody
POLE 3 antibody
Polymerase (DNA directed) epsilon 3 antibody
YBL 1 antibody
Western blot - Anti-DNA Polymerase epsilon antibody (ab105454)
All lanes : Anti-DNA Polymerase epsilon antibody (ab105454) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431) Lane 2 : Human brain tissue lysate - total protein (ab29466) Lane 3 : Human placenta tissue lysate - total protein (ab29745) Lane 4 : Human testis tissue lysate - total protein (ab30257) Lane 5 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa Observed band size: 16 kDa Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.
ICC/IF image of ab105454 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105454, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 1µg/ml.