Anti-POLD2 抗体 (ab38338)
Key features and details
- Rabbit polyclonal to POLD2
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-POLD2 antibody
POLD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to POLD2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Macaque monkey -
免疫原
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ポジティブ・コントロール
- ab38338 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK 293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line), MCF-7 (Human breast adenocarcinoma cell line). and the following Nuclear lysate: HeLa (Human epithelial carcinoma cell line).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab38338の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
The function of the small subunit is not yet clear. -
配列類似性
Belongs to the DNA polymerase delta/II small subunit family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 281991 Cow
- Entrez Gene: 5425 Human
- Entrez Gene: 18972 Mouse
- Entrez Gene: 289758 Rat
- Omim: 600815 Human
- SwissProt: P49004 Cow
- SwissProt: P49005 Human
- SwissProt: O35654 Mouse
see all -
別名
- DNA polymerase delta subunit 2 antibody
- DNA polymerase delta subunit p50 antibody
- DNA polymerase subunit delta 2 antibody
see all
画像
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All lanes : Anti-POLD2 antibody (ab38338) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 :Jurkat whole cell lysate (ab7899)
Lane 4 :A-431 whole cell lysate (ab7909)
Lane 5 :HEK-293 whole cell lysate (ab7902)
Lane 6 : Hep G2 whole cell lysate (ab7900)
Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands. -
ICC/IF image of ab38338 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38338, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab38338 は 4 報の論文で使用されています。
- Bellelli R et al. Pole Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. Mol Cell 70:707-721.e7 (2018). PubMed: 29754823
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Karkhanis V et al. Protein Arginine Methyltransferase 7 Regulates Cellular Response to DNA Damage by Methylating Promoter Histones H2A and H4 of the Polymerase d Catalytic Subunit Gene, POLD1. J Biol Chem 287:29801-14 (2012). WB ; Human . PubMed: 22761421
- Nicolas A et al. Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments. J Virol 84:8871-87 (2010). IF . PubMed: 20573815