製品の概要

  • 製品名
    Anti-DNA polymerase delta p50 antibody
    DNA polymerase delta p50 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA polymerase delta p50
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human DNA polymerase delta p50.

  • ポジティブ・コントロール
    • ab38338 gave a positive result in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK 293 (Human embryonic kidney cell line), HepG2 (Human hepatocellular liver carcinoma cell line), MCF-7 (Human breast adenocarcinoma cell line). and the following Nuclear lysate: HeLa (Human epithelial carcinoma cell line).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

画像

  • All lanes : Anti-DNA polymerase delta p50 antibody (ab38338) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : Jurkat whole cell lysate (ab7899)
    Lane 4 : A431 whole cell lysate (ab7909)
    Lane 5 : HEK293 whole cell lysate (ab7902)
    Lane 6 : HepG2 whole cell lysate (ab7900)
    Lane 7 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab38338 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab38338, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

参考文献

This product has been referenced in:
  • Karkhanis V  et al. Protein Arginine Methyltransferase 7 Regulates Cellular Response to DNA Damage by Methylating Promoter Histones H2A and H4 of the Polymerase d Catalytic Subunit Gene, POLD1. J Biol Chem 287:29801-14 (2012). WB ; Human . Read more (PubMed: 22761421) »
  • Nicolas A  et al. Identification of rep-associated factors in herpes simplex virus type 1-induced adeno-associated virus type 2 replication compartments. J Virol 84:8871-87 (2010). IF . Read more (PubMed: 20573815) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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