製品の概要

  • 製品名Anti-DNA Polymerase beta antibody
    DNA Polymerase beta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Polymerase beta
  • アプリケーション適用あり: IP, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human DNA Polymerase beta.

    (Peptide available as ab27817.)

  • ポジティブ・コントロール
    • This antibody gave a positive control in the following human whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) This antibody gave a positive control in the following mouse embryonic fibroblast cell line lysates: NIH 3T3 whole cell MEF1 whole cell This antibody gave a positive control in the following rat whole cell lysate: PC12 (Rat adrenal pheochromocytoma cell line)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

Anti-DNA Polymerase beta antibody 画像

  • All lanes : Anti-DNA Polymerase beta antibody (ab26343) at 0.5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat whole cell lysate (ab7899)
    Lane 3 : A431 whole cell lysate (ab7909)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 150 kDa (possible cross reactivity),48 kDa (possible cross reactivity),90 kDa (possible cross reactivity).
  • All lanes : Anti-DNA Polymerase beta antibody (ab26343) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Additional bands at : 120 kDa (possible cross reactivity),30 kDa (possible cross reactivity).
  • Image courtesy of Human Protein Atlas ab26343 staining DNA polymerase beta in Human gall bladder, showing a strong nuclear staining pattern in the columnar epithelial cells in contrast with the cells in the lamina propria. Paraffin embedded human gall bladder tissue was incubated with ab26343 (1/150 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab26343 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab26343 stained T47D cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26343 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-DNA Polymerase beta antibody (ab26343) 使用論文

This product has been referenced in:
  • Lokanga RA  et al. Heterozygosity for a hypomorphic Polß mutation reduces the expansion frequency in a mouse model of the Fragile X-related disorders. PLoS Genet 11:e1005181 (2015). WB ; Mouse . Read more (PubMed: 25886163) »
  • Sassa A  et al. Xeroderma Pigmentosum Group A Suppresses Mutagenesis Caused by Clustered Oxidative DNA Adducts in the Human Genome. PLoS One 10:e0142218 (2015). WB ; Human . Read more (PubMed: 26559182) »

See all 7 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (Human Embryonic Kidney cells 293)
Negative control IgG control
Specification Human Embryonic Kidney cells 293
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 30% Formaldehyde
Positive control Input control
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投稿 Nov 18 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 20 µg
Specification HeLa
Treatment siRNA
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Embryonic stem cell)
Loading amount 100 µg
Specification Embryonic stem cell
Gel Running Conditions Reduced Denaturing (8% page gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Apr 27 2012

Thank you for confirming these details and for your cooperation.

As requested, I have issued a free of charge replacement with the order number 1062123.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (Small Cell Lung Cancer)
Total protein in input 2e+006 cells
Specification Small Cell Lung Cancer
Immuno-precipitation step Other - Protein G Dynabeads
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投稿 Nov 19 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small Cell Lung Cancer)
Specification Small Cell Lung Cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Bond ER1
Permeabilization No
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投稿 Nov 18 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Small Cell Lung Cancer)
Loading amount 15 µg
Specification Small Cell Lung Cancer
Treatment POLB overexpressed
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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投稿 Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (1 EBNA, 2 HeLa)
Loading amount 15 µg
Specification 1 EBNA, 2 HeLa
Gel Running Conditions Reduced Denaturing
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 2%
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投稿 Dec 16 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"