Anti-DNA polymerase alpha 抗体 (ab65009)

製品の概要

  • 製品名Anti-DNA polymerase alpha antibody
    DNA polymerase alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA polymerase alpha
  • 特異性This antibody detects endogenous levels of total DNA polymerase alpha protein.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide derived from N-terminal of human DNA polymerase alpha

  • ポジティブ・コントロール
    • Extracts from Jurkat, 293 and HepG2 cells

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab65009 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 169 kDa (predicted molecular weight: 169 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
  • 配列類似性Belongs to the DNA polymerase type-B family.
  • 翻訳後修飾A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686K1672 antibody
    • DNA polymerase alpha 1 catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit antibody
    • DNA polymerase alpha catalytic subunit p180 antibody
    • DNA polymerase alpha p180 subunit antibody
    • DPOLA_HUMAN antibody
    • OTTHUMP00000023089 antibody
    • OTTHUMP00000023090 antibody
    • P180 antibody
    • p180 subunit antibody
    • POLA antibody
    • POLA1 antibody
    • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
    • Polymerase (DNA-directed), alpha (70kD) antibody
    see all

Anti-DNA polymerase alpha antibody 画像

  • All lanes : Anti-DNA polymerase alpha antibody (ab65009) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells
    Lane 2 : Extracts from 293 cells
    Lane 3 : Extracts from HepG2 cells
    Lane 4 : Extracts from HepG2 cells with immunizing peptide

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 169 kDa
    Observed band size : 169 kDa

Anti-DNA polymerase alpha antibody (ab65009) 使用論文

ab65009 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your patience.
For ab57296, the sequence homology of the immunogen with mouse is 83% - which is good.
The lab let me know that for ab65009, the amino acid range of the immunogen is within residues 100-300 of human DNA Polymerase ...

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I am checking with the lab about the homology of the immunogen sequence with mouse. The overall homology between human and mouse is 88%, but some regions align better than others.
I will let you know when I hear back.
What applications are...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"