Anti-DNA polymerase alpha 抗体 (ab57296)

製品の概要

  • 製品名Anti-DNA polymerase alpha antibody
    DNA polymerase alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to DNA polymerase alpha
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: QFSRTGPLCP ACMKATLQPE YSDKSLYTQL CFYRYIFDAE CALEKLTTDH EKDKLKKQFF TPKVLQDYRK LKNTAEQFLS RSGYSEVNLS KLFAGCAVKS , corresponding to amino acids 1363-1463 of Human DNA polymerase alpha

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: Use at a concentration of 1-5 µg/ml.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
    • 配列類似性Belongs to the DNA polymerase type-B family.
    • 翻訳後修飾A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp686K1672 antibody
      • DNA polymerase alpha 1 catalytic subunit antibody
      • DNA polymerase alpha catalytic subunit antibody
      • DNA polymerase alpha catalytic subunit p180 antibody
      • DNA polymerase alpha p180 subunit antibody
      • DPOLA_HUMAN antibody
      • OTTHUMP00000023089 antibody
      • OTTHUMP00000023090 antibody
      • P180 antibody
      • p180 subunit antibody
      • POLA antibody
      • POLA1 antibody
      • Polymerase (DNA directed), alpha 1, catalytic subunit antibody
      • Polymerase (DNA-directed), alpha (70kD) antibody
      see all

    Anti-DNA polymerase alpha antibody 画像



    • Predicted band size : 166 kDa
      DNA polymerase alpha antibody (ab57296) at 1ug/lane + HeLa cell lysate at 25ug/lane.

    Anti-DNA polymerase alpha antibody (ab57296) 使用論文

    ab57296 has not yet been referenced specifically in any publications.

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