Anti-DNA Ligase III 抗体 (ab97404)

製品の概要

  • 製品名
    Anti-DNA Ligase III antibody
    DNA Ligase III 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Ligase III
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 116-403 of Human DNA Ligase III (NP_039269).

  • ポジティブ・コントロール
    • HepG2 whole cell lysate; A549 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/250.

ターゲット情報

  • 機能
    Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • 組織特異性
    Testis, thymus, prostate and heart.
  • 配列類似性
    Belongs to the ATP-dependent DNA ligase family.
    Contains 1 BRCT domain.
    Contains 1 PARP-type zinc finger.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D11Wsu78e antibody
    • DNA ligase antibody
    • DNA ligase 3 antibody
    • DNA ligase III antibody
    • DNLI3_HUMAN antibody
    • LIG 3 antibody
    • LIG2 antibody
    • LIG3 antibody
    • Ligase II, DNA, ATP dependent antibody
    • Ligase III DNA ATP dependent antibody
    • MGC78176 antibody
    • OTTMUSP00000000875 antibody
    • Polydeoxyribonucleotide synthase [ATP] 3 antibody
    • Polydeoxyribonucleotide synthase [ATP] antibody
    • Polydeoxyribonucleotide synthase antibody
    • RP23-316C11.6 antibody
    see all

画像

  • Anti-DNA Ligase III antibody (ab97404) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 113 kDa

    7.5% SDS-PAGE

     

    Secondary antibody - anti-rabbit HRP (ab6721)

  • ab97404, at 1/100 dilution, staining DNA Ligase III in paraffin-embedded A549 xenograft by Immunohistochemistry.

参考文献

ab97404 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting Abcam regarding ab97404.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions that may...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain, Heart, Lung and Kidney)
Loading amount
40 µg
Specification
Brain, Heart, Lung and Kidney
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 May 16 2012

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Thank you for contacting us and for keeping us updated about the situation. I am sorry to hear that the suggestions from my colleague did not help improve the results.

At this time we have only tested ab97404 with human lysates, and though we...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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