Anti-DNA Ligase III 抗体 (ab97404)


  • 製品名Anti-DNA Ligase III antibody
    DNA Ligase III 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Ligase III
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 116-403 of Human DNA Ligase III (NP_039269).

  • ポジティブ・コントロール
    • HepG2 whole cell lysate; A549 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates.



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab97404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 113 kDa.
IHC-P 1/100 - 1/250.


  • 機能Interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.
  • 組織特異性Testis, thymus, prostate and heart.
  • 配列類似性Belongs to the ATP-dependent DNA ligase family.
    Contains 1 BRCT domain.
    Contains 1 PARP-type zinc finger.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D11Wsu78e antibody
    • DNA ligase antibody
    • DNA ligase 3 antibody
    • DNA ligase III antibody
    • DNLI3_HUMAN antibody
    • LIG 3 antibody
    • LIG2 antibody
    • LIG3 antibody
    • Ligase II, DNA, ATP dependent antibody
    • Ligase III DNA ATP dependent antibody
    • MGC78176 antibody
    • OTTMUSP00000000875 antibody
    • Polydeoxyribonucleotide synthase [ATP] 3 antibody
    • Polydeoxyribonucleotide synthase [ATP] antibody
    • Polydeoxyribonucleotide synthase antibody
    • RP23-316C11.6 antibody
    see all

Anti-DNA Ligase III antibody 画像

  • Anti-DNA Ligase III antibody (ab97404) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 113 kDa

    7.5% SDS-PAGE


    Secondary antibody - anti-rabbit HRP (ab6721)

  • ab97404, at 1/100 dilution, staining DNA Ligase III in paraffin-embedded A549 xenograft by Immunohistochemistry.

Anti-DNA Ligase III antibody (ab97404) 使用論文

ab97404 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam regarding ab97404.

I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I would like to make some suggestions that may...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Brain, Heart, Lung and Kidney)
Loading amount 40 µg
Specification Brain, Heart, Lung and Kidney
Gel Running Conditions Reduced Denaturing (4-15% gradient)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 May 16 2012

Thank you for your email.

I've just added the discount code toour internal notes of your review, so it is now active and can be redeemed for a free antibody.

Please let me know if there is anything else that we can do for you. Have ...

Read More

Thank you for your reply.

An account was automatically generated for your email addressin order for us to correspond with you. You can change your password and access this account by going to "sign in" onthe http://www.abcam.comwebp...

Read More

Thank you for contacting us and for keeping us updated about the situation. I am sorry to hear that the suggestions from my colleague did not help improve the results.

At this time we have only tested ab97404 with human lysates, and though we...

Read More