保存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー Preservative: None
Constituents: 2% Sucrose, PBS
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精製度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 102 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay 1:62500.
機能 DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
配列類似性 Belongs to the ATP-dependent DNA ligase family.
Information by UniProt
DNA ligase 1 antibody
DNA ligase I antibody
Anti-DNA Ligase I antibody 画像
Western blot - DNA Ligase I antibody (ab76232)
Anti-DNA Ligase I antibody (ab76232) 使用論文
This product has been referenced in:
et al. Heterozygosity for a hypomorphic Polß mutation reduces the expansion frequency in a mouse model of the Fragile X-related disorders. PLoS Genet 11:e1005181 (2015).
Read more (PubMed: 25886163) »
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"