製品の概要

  • 製品名Anti-DNA Ligase I antibody [10H5]
    DNA Ligase I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10H5] to DNA Ligase I
  • アプリケーション適用あり: WB, IP, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant human DNA Ligase I protein encoding amino acids 1-919 purified from baculovirus infected Sf9 cells.

  • ポジティブ・コントロール
    • MCF7 cells and recombinant protein.
  • 特記事項DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: None
    Constituents: 10mM PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名10H5
  • ミエローマNS1
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/3000. Predicted molecular weight: 101 kDa.
IP Use at an assay dependent concentration.
Flow Cyt 1/50.
ICC/IF 1/50.

ターゲット情報

  • 機能DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
  • 配列類似性Belongs to the ATP-dependent DNA ligase family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DNA ligase 1 antibody
    • DNA ligase I antibody
    • DNLI1_HUMAN antibody
    • LIG 1 antibody
    • lig1 antibody
    • Ligase I DNA ATP dependent antibody
    • MGC117397 antibody
    • MGC130025 antibody
    • Polydeoxyribonucleotide synthase [ATP] 1 antibody
    see all

Anti-DNA Ligase I antibody [10H5] 画像

  • ab615 at 1/150 staining normal human primary fibroblasts by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 16 hours. A FITC conjugated goat polyclonal antibody was used as the secondary.

    See Abreview

  • ab615 (2µg/ml) staining DNA Ligase 1 in human colonic mucosa, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of mucosal epithelium .
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Overlay histogram showing Jurkat cells stained with ab615 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab615, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-DNA Ligase I antibody [10H5] (ab615) 使用論文

This product has been referenced in:
  • Gu L  et al. A PCNA-derived cell permeable peptide selectively inhibits neuroblastoma cell growth. PLoS One 9:e94773 (2014). ICC/IF ; Human . Read more (PubMed: 24728180) »
  • Sousa MM  et al. An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells. PLoS One 8:e55493 (2013). WB . Read more (PubMed: 23405159) »

See all 10 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (MCF-7 breast cancer cells)
Negative control See figure
Specification MCF-7 breast cancer cells
Detection step Semiquantitative PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Positive control See figure
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Verified customer

投稿 May 16 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (fibroblast)
Loading amount 10 µg
Specification fibroblast
Treatment siRNA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Sep 18 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Normal Fibroblast Primary)
Specification Normal Fibroblast Primary
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jul 27 2007

As this antibody was purified from tissue culture supernatant, there is probably some cow IgG present from the serum. However, the antibody present that recognizes DNA ligase has not been modified. The concentration is at 1 mg/ml.

We have not observed any cross-reactivity with the other human DNA ligases.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"