DNA Isolation & Bisulfite Conversion Kit (ab117124)

製品の概要

  • 製品名DNA Isolation & Bisulfite Conversion Kit
    DNA modification キット 製品一覧
  • テスト
    1 x 40 test
  • サンプルの種類
    Serum, Plasma, Adherent cells, Suspension cells
  • 全工程の試験時間
    2h 20m
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: all Mammals
  • 製品の概要

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It is well demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases.

    Most methods for DNA methylation detection require a prior bisulfite-based DNA modification. Bisulfite-based DNA modification is used to discrimate between cytosine and methylated cytosine, in which DNA is treated with bisulfite salt to convert cytosine residues to uracil in a single-stranded DNA, while methylated cytosine remains the same.
    DNA purification & modification Kit (ab117124) allows the user to isolate and modify DNA efficiently. The entire procedure can be completed within 2 hours and 20 minutes is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.

    Not sure if this is the right kit for you? Check out our bisulfite modification guide for more information.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab117124 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    プロトコール

    DNA Isolation & Bisulfite Conversion Kit (ab117124) 使用論文

    ab117124 has not yet been referenced specifically in any publications.

    Product Wall

    25 ul serum or 50 ul of plasma is fine for using with this kit. The EBM3 buffer can be directly added into the serum/plasma sample. 25 ul of serum would be fine for obtaining modified DNA. However we are not sure if the modified DNA can be obtained fro...

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    Thank you for contacting us.

    Unfortunately, we do not carry DNA purification columns, but we do carry a kit:

    EpiSeeker DNA purification & modification Kit (ab117124) [ http://www.abcam.com/EpiSeeker-DNA-purification--modificati...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"