Anti-DNA Gyrase B 抗体 (ab75596)

製品の概要

  • 製品名Anti-DNA Gyrase B antibody
    DNA Gyrase B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Gyrase B
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Escherichia coli , Staphylococcus aureus
  • 免疫原

    Purified E. coli Gyr B protein (over expressed from E. coli)

  • ポジティブ・コントロール
    • E. coli cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)This antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.

Can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed gyrase in cell extracts. The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

ターゲット情報

  • 関連性DNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
  • 参照データベース
    • 別名
      • acrB antibody
      • DNA gyrase subunit B antibody
      • himB antibody
      • hisU antibody
      • nalC antibody
      • parA antibody
      • pcbA antibody
      see all

    Anti-DNA Gyrase B antibody 画像

    • All lanes : Anti-DNA Gyrase B antibody (ab75596) at 1/1000 dilution

      Lane 1 : E. coli cell lysate (Gyrase A, whole protein) at 0.1 µg
      Lane 2 : E. coli cell lysate (Gyrase A, whole protein) at 0.5 µg
      Lane 3 : E. coli cell lysate (Gyrase A, whole protein) at 1 µg
      Lane 4 : E. coli cell lysate (Gyrase B, whole protein) at 0.1 µg
      Lane 5 : E. coli cell lysate (Gyrase B, whole protein) at 0.5 µg
      Lane 6 : E. coli cell lysate (Gyrase B, whole protein) at 1 µg
      Lane 7 : E. coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
      Lane 8 : E. coli cell lysate (Gyrase A, A59 domain) at 0.1 µg

      Secondary
      goat anti rabbit Igs at 1/5000 dilution

      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Anti-DNA Gyrase B antibody (ab75596) 使用論文

    ab75596 has not yet been referenced specifically in any publications.

    Product Wall



    This polyclonal antibody ab75596 it will recognise all gyrase/fragments whole protein or separate domains.
    If you wish to use a more specific antibody which recognises the gyrase B43 and 24 domains, we carry the monclonal antibody [7D3] w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"