製品の概要

  • 製品名Anti-DNA Gyrase B antibody [7D3]
    DNA Gyrase B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [7D3] to DNA Gyrase B
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Escherichia coli
  • 免疫原

    Purified Escherichia coli DNA Gyrase B protein (over expressed from Escherichia coli)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purified from tissue culture supernatant on a Protein A column using the low salt method. The antibody is purified to >95% purity as judged by SDS polyacrylamide gel electrophoresis.
  • ポリ/モノモノクローナル
  • クローン名7D3
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000. The antibody can be diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts. It can be used in Western Blots to detect purified over expressed Gyrase B and unexpressed Gyrase in cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性DNA Gyrase B belongs to the type II topoisomerase family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
    • 参照データベース
      • 別名
        • acrB antibody
        • DNA gyrase subunit B antibody
        • himB antibody
        • hisU antibody
        • nalC antibody
        • parA antibody
        • pcbA antibody
        see all

      Anti-DNA Gyrase B antibody [7D3] 画像

      • All lanes : Anti-DNA Gyrase B antibody [7D3] (ab75586) at 1/1000 dilution

        Lane 1 : E. coli cell lysate (B24 domain of DNA Gyrase B protein)
        Lane 2 : E. coli cell lysate (B43 domain of DNA Gyrase B protein)
        Lane 3 : E. coli cell lysate (whole DNA Gyrase B protein)

        Lysates/proteins at 200 µg per lane.

        Secondary
        HRP labelled rabbit anti mouse IgGs at 1/5000 dilution

        Observed band size : 90 kDa (why is the actual band size different from the predicted?)

      Anti-DNA Gyrase B antibody [7D3] (ab75586) 使用論文

      ab75586 has not yet been referenced specifically in any publications.

      Product Wall

      There are currently no Abreviews or Questions for ab75586.
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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"