製品の概要

  • 製品名Anti-DNA Gyrase A antibody
    DNA Gyrase A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DNA Gyrase A
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Escherichia coli , Staphylococcus aureus
  • 免疫原

    Purified E. coli DNA Gyrase A protein (over expressed from E. coli)

  • ポジティブ・コントロール
    • E.coli cell lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.01% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)The antibody is isolated from rabbit blood and further purified on a Protein A column using the low salt method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報WB: 1/1000.
    Can be used in Western Blots to detect purified over expressed Gyrase A and unexpressed gyrase in cell extracts.
    The antibody is diluted 1/1000 for detection of purified proteins and 1/200 for cell extracts.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性DNA Gyrase A belongs to the topoisomerase gyrA/parC subunit family. DNA gyrase negatively supercoils closed circular double stranded DNA in an ATP dependent manner and also catalyzes the interconversion of other topological isomers of double stranded DNA rings, including catenanes and knotted rings. It is made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer. When the enzyme transiently cleaves DNA a phosphotyrosine bond is formed between the gyrA and DNA.
    • 参照データベース
      • 別名
        • DNA gyrase subunit A antibody
        • GyrA antibody
        • HisW antibody
        • NalA antibody
        • ParD antibody
        see all

      Anti-DNA Gyrase A antibody 画像

      • All lanes : Anti-DNA Gyrase A antibody (ab75594) at 1/1000 dilution

        Lane 1 : E coli cell lysate (Gyrase A, A59 domain) at 0.1 µg
        Lane 2 : E coli cell lysate (Gyrase B, B43 domain) at 0.1 µg
        Lane 3 : E coli cell lysate (Gyrase B, whole protein) at 1 µg
        Lane 4 : E coli cell lysate (Gyrase B, whole protein) at 0.5 µg
        Lane 5 : E coli cell lysate (Gyrase B, whole protein) at 0.1 µg
        Lane 6 : E coli cell lysate (Gyrase A, whole protein) at 1 µg
        Lane 7 : E coli cell lysate (Gyrase A, whole protein) at 0.5 µg
        Lane 8 : E coli cell lysate (Gyrase A, whole protein) at 0.1 µg

        Secondary
        goat anti rabbit Igs at 1/5000 dilution

        Observed band size : 97 kDa (why is the actual band size different from the predicted?)

      Anti-DNA Gyrase A antibody (ab75594) 使用論文

      This product has been referenced in:
      • Aedo S & Tse-Dinh YC Isolation and quantitation of topoisomerase complexes accumulated on escherichia coli chromosomal DNA. Antimicrob Agents Chemother 56:5458-64 (2012). WB . Read more (PubMed: 22869559) »

      See 1 Publication for this product

      Product Wall

      Thank you for contacting us.

      Most of our antibodies are stable at -20C for up to five years. For short term storage we recommend storing at 4C. It is also important to aliquot them in order to minimize damage due to freezing and thawing.
      Read More

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"