Biotin Anti-DMPO Nitrone Adduct 抗体 [N1664A] (ab110430)
Key features and details
- Biotin Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
- Suitable for: IP, ICC/IF, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Biotin Anti-DMPO Nitrone Adduct antibody [N1664A]
DMPO Nitrone Adduct 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [N1664A] to DMPO Nitrone Adduct -
由来種
Mouse -
標識
Biotin -
特異性
Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA. -
アプリケーション
適用あり: IP, ICC/IF, WBmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to DMPO Nitrone Adduct conjugated to ovalbumin. 5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
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ポジティブ・コントロール
- ICC/IF: RAW 264.7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N1664A -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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特記事項 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ターゲット情報
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関連性
For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts. -
別名
- 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
画像
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RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells stained for DMPO Nitrone Adduct using the unconjugated version of ab110430 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Mouse IgG (Green). Counterstain: DAPI (Blue) nuclear stain.
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All lanes : Unconjugated version of ab110430 at 1/200 dilution
Lane 1 : 10 um hemoglobin
Lane 2 : 10 um hemoglobin + 100 uM HOCl
Lane 3 : 10 um hemoglobin + 500 uM HOCl
Lane 4 : 10 um hemoglobin + 1000 uM HOCl
Lane 5 : 10 um hemoglobin + 20 mM DMPO
Lane 6 : 10 um hemoglobin + 100 uM HOCl + 20 mM DMPO
Lane 7 : 10 um hemoglobin + 500 uM HOCl + 20 mM DMPO
Lane 8 : 10 um hemoglobin + 1000 uM HOCl + 20 mM DMPO
Lane 9 : 10 um hemoglobin + 100 mM DMPO
Lane 10 : 10 um hemoglobin + 100 uM HOCl + 100 mM DMPO
Lane 11 : 10 um hemoglobin + 500 uM HOCl + 100 mM DMPO
Lane 12 : 10 um hemoglobin + 1000 uM HOCl + 100 mM DMPO
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab110430 は 1 報の論文で使用されています。
- Hamasaki T et al. Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. PLoS One 12:e0171192 (2017). ICC/IF ; Human . PubMed: 28182635