Anti-DMPO Nitrone Adduct 抗体 [N1664A] (ab104902)
Key features and details
- Mouse monoclonal [N1664A] to DMPO Nitrone Adduct
- Suitable for: WB, ELISA, ICC/IF, IP
- Reacts with: Species independent
- Isotype: IgG1
Related conjugates and formulations
製品の概要
-
製品名
Anti-DMPO Nitrone Adduct antibody [N1664A]
DMPO Nitrone Adduct 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [N1664A] to DMPO Nitrone Adduct -
由来種
Mouse -
特異性
Detects ~90kDa. Recognizes DMPO, DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not cross-react with non-adducted proteins or DNA. -
アプリケーション
適用あり: WB, ELISA, ICC/IF, IPmore details -
種交差性
交差種: Species independent -
免疫原
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to Ovalbumin.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), Tris buffered saline -
Concentration information loading...
-
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
N1664A -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104902の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 - 10 µg/ml.
|
|
ELISA |
Use a concentration of 1 - 10 µg/ml.
|
|
ICC/IF |
Use a concentration of 1 - 10 µg/ml.
|
|
IP |
Use at an assay dependent dilution. Follow the method described by Chatterjee, S. et. al. PubMed 19049863.
|
特記事項 |
---|
WB
Use a concentration of 1 - 10 µg/ml. |
ELISA
Use a concentration of 1 - 10 µg/ml. |
ICC/IF
Use a concentration of 1 - 10 µg/ml. |
IP
Use at an assay dependent dilution. Follow the method described by Chatterjee, S. et. al. PubMed 19049863. |
ターゲット情報
-
関連性
For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts. -
別名
- 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody
画像
-
Immuncytochemistry/Immunofluorescence analysis of Mouse macrophage cells labelling DMPO Nitrone Adduct with ab104902 at 10μg/mL. Left - with DMPO, Right - no DMPO. Top to bottom - DMPO, DAPI, overlay.
-
All lanes : Anti-DMPO Nitrone Adduct antibody [N1664A] (ab104902) at 10 µg/ml
Lane 1 : 10µM Hb
Lane 2 : 10µM Hb + 100µM HOCL
Lane 3 : 10µM Hb + 500µM HOCL
Lane 4 : 10µM Hb + 1000µM HOCL
Lane 5 : 10µM Hb with 20mM DMPO
Lane 6 : 10µM Hb + 100µM HOCL with 20mM DMPO
Lane 7 : 10µM Hb + 500µM HOCL with 20mM DMPO
Lane 8 : 10µM Hb + 1000µM HOCL with 20mM DMPO
Lane 9 : 10µM Hb with 100mM DMPO
Lane 10 : 10µM Hb + 100µM HOCL with 100mM DMPO
Lane 11 : 10µM Hb + 500µM HOCL with 100mM DMPO
Lane 12 : 10µM Hb + 1000µM HOCL with 100mM DMPO
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab104902 は 3 報の論文で使用されています。
- Chen T et al. Edonerpic maleate regulates glutamate receptors through CRMP2- and Arc-mediated mechanisms in response to brain trauma. Cell Death Discov 8:95 (2022). PubMed: 35246523
- Lozoya OA et al. Single Nucleotide Resolution Analysis Reveals Pervasive, Long-Lasting DNA Methylation Changes by Developmental Exposure to a Mitochondrial Toxicant. Cell Rep 32:108131 (2020). PubMed: 32937126
- Sun L et al. Targeted DNA damage at individual telomeres disrupts their integrity and triggers cell death. Nucleic Acids Res 43:6334-47 (2015). PubMed: 26082495