製品の概要

  • 製品名Anti-DMPO Nitrone Adduct antibody
    DMPO Nitrone Adduct 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to DMPO Nitrone Adduct
  • アプリケーション適用あり: WB, ELISAmore details
  • 免疫原

    5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline-N-oxide conjugated to Ovalbumin.

  • 特記事項


    Measurement of protein radicals is now possible with the development of the "spin trap immunoassay" (please see refs 1,2). Compared to traditional techniques, immunodetection of spin trap products allows a higher level of sensitivity and throughput with greatly reduced sample consumption.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab23702 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 17 kDa.
ELISA 1/1000 - 1/5000.

ターゲット情報

  • 関連性For measurement of protein radicals; some proteins with endogenous peroxidase activity are especially susceptible to forming DMPO adducts.
  • 別名
    • 55 dimethyl 1 pyrroline N oxide nitrone adduct antibody

Anti-DMPO Nitrone Adduct antibody 画像

  • All lanes : Anti-DMPO Nitrone Adduct antibody (ab23702)

    Lane 1 : Metmyoglobin at 3 µl
    Lane 2 : Metmyoglobin-DMPO at 3 µl
    Lane 3 : Metmyoglobin-DMPO at 5 µl
    Lane 4 : Metmyoglobin-DMPO at 10 µl


    Observed band size : 17 kDa (why is the actual band size different from the predicted?)

Anti-DMPO Nitrone Adduct antibody (ab23702) 使用論文

This product has been referenced in:
  • Gomez-Mejiba SE  et al. Immuno-spin trapping of protein and DNA radicals: "tagging" free radicals to locate and understand the redox process. Free Radic Biol Med 46:853-65 (2009). ELISA . Read more (PubMed: 19159679) »
  • Schildknecht S  et al. Acetaminophen inhibits prostanoid synthesis by scavenging the PGHS-activator peroxynitrite. FASEB J 22:215-24 (2008). WB . Read more (PubMed: 17724253) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"