製品の概要

  • 製品名Anti-DmIh antibody
  • 製品の詳細
    Mouse polyclonal to DmIh
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Vector coding for a partial recombinant fusion protein, corresponding to C terminal amino acids 1252-1327 of Fruit fly (Drosophila melanogaster) DmIh. HQKDEQLSNP ESNTITAVVN ALAAEADDCK DDDMDLKENL LHGSESSIA EPVQTIREGL PRPRSGEFRA LFEGNTP.

  • 特記事項

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 147 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性DmIh is an intracellular cyclic nucleotide activated cation channel.
  • 細胞内局在Plasma membrane
  • 参照データベース
    • 別名
      • DMIH antibody
      • HCN4 antibody
      • Ih antibody
      • Putative Ih channel antibody
      • Putative voltage and cyclic nucleotide gated ion channel antibody
      see all

    Anti-DmIh antibody 画像

    • All lanes : Anti-DmIh antibody (ab52694) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli containing 50 - 100ng of a tagged fusion protein of an irrelevant antigen
      Lane 2 : Total protein extract from E. coli containing 50 - 500ng of the antigen (tagged fusion protein)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit anti-mouse IgG + IgM HRP conjugated, at 1/5000 dilution

      Predicted band size : 147 kDa

    Anti-DmIh antibody (ab52694) 使用論文

    ab52694 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab52694.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"