製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to DMC1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Zebrafish非交差種: Arabidopsis thaliana
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 168-316 of Human DMC1 (NP_008999).

  • ポジティブ・コントロール
    • Raji whole cell lysate; 293T, A431, H1299, HeLa, HepG2 and Molt-4 cell lysates

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 40% Glycerol, 1X PBS, pH 7.0
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 38 kDa.

ターゲット情報

  • 機能
    May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
  • 配列類似性
    Belongs to the RecA family. DMC1 subfamily.
    Contains 1 HhH domain.
  • 細胞内局在
    Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Disrupted meiotic cDNA 1 homolog antibody
    • Disrupted meiotic cDNA 1, yeast, homolog of antibody
    • dJ199H16.1 antibody
    • DMC 1 antibody
    • DMC1 antibody
    • DMC1 dosage suppressor of mck1 homolog antibody
    • DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
    • DMC1 homologue antibody
    • DMC1_HUMAN antibody
    • DMC1H antibody
    • DNA meiotic recombinase 1 antibody
    • HsLim15 antibody
    • LIM15 antibody
    • Meiotic recombination protein DMC1/LIM15 homolog antibody
    • MGC150472 antibody
    • MGC150473 antibody
    see all

画像

  • Anti-DMC1 antibody (ab97313) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 38 kDa

プロトコール

参考文献

ab97313 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
900 µg
Gel Running Conditions
Reduced Denaturing (12%, Laemmli)
Sample
Arabidopsis thaliana Tissue lysate - whole (closed flower buds)
Specification
closed flower buds
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Natasha Elina

Verified customer

投稿 Mar 04 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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