製品の概要

  • 製品名Anti-DMC1 antibody [2H12/4]
    DMC1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2H12/4] to DMC1
  • 特異性Specific for DMC1 - does not cross-react with the related protein Rad51. The antibody was tested on mouse meiotic chromosomes. It allowed localisation of the Dmc1 protein as numerous foci in the Leptotene and Zygotene stages of meiotic prophase, and fewer foci in the early Pachytene stage of meiotic prophase.
  • アプリケーション適用あり: Flow Cyt, IP, WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant fusion protein (Human).

  • ポジティブ・コントロール
    • ICC:mouse meiotic chromosomes

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名2H12/4
  • ミエローマSp2/0-Ag14
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
ICC/IF 1/100. Fix cells using a 4% paraformaldehyde solution and permeabilize using SDS (0.03% in a 4% paraformaldehyde solution). Incubate antibody overnight.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
  • 配列類似性Belongs to the RecA family. DMC1 subfamily.
    Contains 1 HhH domain.
  • 細胞内局在Nucleus. Chromosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Disrupted meiotic cDNA 1 homolog antibody
    • Disrupted meiotic cDNA 1, yeast, homolog of antibody
    • dJ199H16.1 antibody
    • DMC 1 antibody
    • DMC1 antibody
    • DMC1 dosage suppressor of mck1 homolog antibody
    • DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast) antibody
    • DMC1 homologue antibody
    • DMC1_HUMAN antibody
    • DMC1H antibody
    • DNA meiotic recombinase 1 antibody
    • HsLim15 antibody
    • LIM15 antibody
    • Meiotic recombination protein DMC1/LIM15 homolog antibody
    • MGC150472 antibody
    • MGC150473 antibody
    see all

Anti-DMC1 antibody [2H12/4] 画像

  • Overlay histogram showing HeLa cells stained with ab11054 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11054, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-DMC1 antibody [2H12/4] (ab11054) 使用論文

This product has been referenced in:
  • Hopkins J  et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). ICC/IF ; Mouse . Read more (PubMed: 24992337) »
  • Sobinoff AP  et al. Damaging legacy: maternal cigarette smoking has long-term consequences for male offspring fertility. Hum Reprod 29:2719-35 (2014). IHC ; Mouse . Read more (PubMed: 25269568) »

See all 7 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

投稿 Nov 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (adult testes)
Specification adult testes
Fixative 4% PFA
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Qing Wen

Verified customer

投稿 Mar 18 2013

Thank you for your enquiry.

I am sorry to hear the replacement antibody is still not working as expected. I would be happy to send you different Dmc1 antibody of your choice. So I can process your request, please include the purchase order nu...

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Merci de nous avoir contactés. Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Comme convenu j'ai mis en place l'envoi d'une unité de remplacement avec un lot différent, le numéro de commande de rem...

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Thank you for contacting us and I'm sorry to hear that this antibody is giving you some trouble. I think that boiling will more properly denature the protein and allow it to move more efficiently through the gel. To denature, use a loading buffer...

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I'm very sorry to hear you are still having no staining with the recent set of experiments, I appreciate the effort you have put to make the experiments work and cannot understand why the antibody did not work for you. We have not received any other ...

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I am pleased to inform you that I have received just this morning a detailed protocol for staining with ab11054. The antibody has been very satisfactory in detecting the Dmc1 protein on mouse meiotic chromosomes. The meiotic chromosomes spreads wer...

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I'm very sorry for the delay in finding out more information to help you and to hear you are experiencing problems with two of our antibodies. I have been informed that ab36453 has been used in the following reference: Geddis AE & Kaushansky K. Meg...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (testis)
Loading amount 40 µg
Specification testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 May 25 2006

Thank you for your enquiry. I am sorry to hear that your customer has been having difficulties with this antibody. Quality is important to Abcam. Your customer is using a western blotting approach that I would largely recommend. I would be very int...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"