製品の概要

  • 製品名Anti-Dlx5 antibody [EPR4488]
    Dlx5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4488] to Dlx5
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Dlx5 aa 150-250.

  • ポジティブ・コントロール
    • WB: C6, RAW264.7, PC12, and HeLa cell lysates. IHC-P: Human brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109737 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000.

For unpurified use at 1/50 - 1/100.

WB 1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP 1/150.

For unpurified use at 1/10 - 1/100.

IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Antigen retreval recommended; heat up to 98 degrees C, below boiling, and then let cool for 10-20 min

ターゲット情報

Anti-Dlx5 antibody [EPR4488] 画像

  • Lanes 1 - 2 : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/5000 dilution (purified)
    Lane 3 : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/10000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 32 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/5000 dilution (purified)

    Lane 1 : HeLa cell lysate
    Lane 2 : U87-MG cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 32 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Dlx5 antibody [EPR4488] (ab109737) at 1/1000 dilution (unpurified)

    Lane 1 : C6 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : PC12 cell lysate
    Lane 4 : HeLa cell lysates

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 32 kDa
  • ab109737 (purified) at 1/150 immunoprecipitating SFTPA1 + SFTPA2 in PC-12 whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/10,000) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human astrocytoma tissue labelling Dlx5 with purified ab109737 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain tissue labelling Dlx5 with unpurified ab109737 at 1/100.

  • Immunocytochemistry/Immunofluorescence analysis of C6 (rat glioma) cells labelling Dlx5 with purified ab109737 at 1/1000. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Anti-Dlx5 antibody [EPR4488] (ab109737) 使用論文

This product has been referenced in:
  • Rattanasopha S  et al. Absent expression of the osteoblast-specific maternally imprinted genes, DLX5 and DLX6, causes split hand/split foot malformation type I. J Med Genet 51:817-23 (2014). WB ; Human . Read more (PubMed: 25332435) »

See 1 Publication for this product

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